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The antibacterial potential of lemon (Citrus limon) peel extract on Escherichia coli (ATCC # 25922).


Kim Marie P. Capilitan,
John Tristan R. Gullas,
Peter John M. Hicban,
Sofia Pauline G. Mercado,
Shelsey Jul I. Ytol,
Maricel  B. Flores

Related Institution

College of Arts and Sciences - Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
February 2020


   The study was conducted to determine the antibacterial potential of lemon peel (Citrus limon) extract by measuring the zone of inhibition in three different concentrations of 25%, 50% and 100% against Escherichia coli (ATCC no. 25922). One-way ANOVA was the statistical treatment used. The positive control used was Ofloxacin paper discs while the negative control was 95% ethanol. Each experimental and control groups were allocated with five petri dishes streaked with Escherichia coli (ATCC no. 25922), and five paper discs dipped in the solutions of the experimental and control groups were used for treatment. After 24 hours of incubation at 37 degrees Celsius, all of the zones of inhibition were subject to measurement and the results were tabulated. This study used a completely randomized design (CRD).

   The results of the experiment are as follows: for the 25% and 50% concentrations no zone of inhibition was formed while the 100% concentration showed a zone of inhibition ranging from 7mm to 9mm. The 95% ethanol did not show any Zone of Inhibition, but the Ofloxacin successfully showed zones of inhibition ranging from 25mm to 33mm. The extract was not able to reach the required zone of inhibition in the Kirby Bauer Standard Chart, which has a resistant zone of inhibition of 15mm and a susceptible zone of inhibition of 21mm. Therefore, Escherichia coli is not susceptible to Citrus limon peel extract. One-Way ANOVA revealed that on average, the log zone of inhibition exhibited by the Ofloxacin treatment group was significantly bigger compared with the 100% lemon peel extract, F(1,48) = 2632.469, p < .05.

   In conclusion, the result showed that the lemon peel extract does not have antibacterial effect on E. coli (ATCC no. 25922). Preparatory measures, specifically sun drying of the lemon peels, for the experiment may have an effect on the results. Indoor drying is recommended instead of sun drying to avoid reduction of the antibacterial potential of the peel.


1. Badayos, A. . "E.coli found in water at evacuation centers." Sunstar , September 2018. Retrieved from: https://www.sunstar.com.ph/article/1765843
2. Castanie-Cornet, M. , Penfound, T. A. , Smith, D. , Elliott, J. F. , Foster, J. W. . "Control of acid resistance in escherichia coli." Journal of Bacteriology 181(11): 3525-3535, 1999. Retrieved from: http://doi:10.1128/jb.181.11.3525-3535.1999.
3. Centers for Disease Control and Prevention(2020, June 9). E. coli and food safety.. . Retrieved August 10, 2020, from: https://www.cdc.gov/foodsafety/communication/ecoli-and-foodsafety.html
4. Clements, A. , Young, J. C. , Constantinou, N. , Frankel, G. . "Infection strategies of enteric pathogenic Escherichia coli." Gut Microbes 3(2): 71-87, 2012. Retrieved from: http://doi:10.4161/gmic.19182
5. Dhanavade, M. J. , Jalkute, C. B. , Ghosh, J. S. , Sonawane, K. D. . "Study antimicrobial activity of lemon (Citrus lemon L.) peel extract." British Journal of Pharmacology and Toxicology 2(3): 119-122, 2011. Retrieved from: https://www.researchgate.net/publication/236217959_Study_Antimicrobial_Activity_of_Lemon_Citrus_lemon_L_Peel_Extract
6. Djenane, D. . "Chemical profile, antibacterial and antioxidant activity of algerian citrus essential oils and their application in sardinapilchardus" Foods 4(4): 208-228, 2015. Retrieved from: http://doi: 10.3390/foods4020208
7. Exner, M. , Bhattacharya, S. , Christiansen, B. , Gebel, J. , Goroncy-Bermes, P. , Hartemann, P. , Trautmann, M. . "Antibiotic resistance: What is so special about multidrug-resistant Gram negative bacteria?" GMS Hygiene and Infecetion Control 12, 05, April 2017. Retrieved from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388835/
8. Gorden, J. , Small, P. L. . "Acid resistance in enteric bacteria" Infection and Immunity 61(1): 1993. Retrieved from: http://doi: 10.1128/iai.61.1.364-367.1993
9. Górniak, I. , Bartoszewski, R. , Króliczewski, J. . "Comprehensive review of antimicrobial activities of plant flavonoids" Phytochemistry Reviews 18(1): 241–272, 2018. Retrieved from: http://doi:10.1007/s11101-018-9591-z
10. Guentzel, M. . Chapter 26. Medical Microbiology(4th ed.) Galveston, Texas: University of Texas Medical Branch at Gavelston, 1996. 1-2. . Retrieved from: https://pubmed.ncbi.nlm.nih.gov/21413290/
11. Kanjee, U. , Houry, W. A. . "Mechanisms of acid resistance in Escherichia coli" Annual Review of Microbiology 67(1): 2013. Retrieved from: http://doi:10.1146/annurev-micro-092412-155708
12. Ling, A. L. , Yasir, S. , Matanjun, P. , Bakar, M. F. . "Effect of different drying techniques on the phytochemical content and antioxidant activity of Kappaphycus alvarezii." Journal of Applied Phycology 27(4): 1717-1723, 2014. Retrieved from: http://doi:10.1007/s10811-014-0467-3
13. Madappa, T. . (). Escherichia coli (E coli) infections.. . Retrieved February 6, 2019, from: https://emedicine.medscape.com/article/217485-overview
14. Moosavy, M. H. , Hassanzadeh, P. , Mohammadzadeh, E. , Mahmoudi, R. , Khatibi, S. A. , Mardani, K. . "Antioxidant and antimicrobial activities of essential oil of lemon (Citrus limon) peel in vitro and ina food model" Journal of Food Quality and Hazards Control 4, 42-48, 2017. Retrieved from: http://jfqhc.ssu.ac.ir/article-1-335-en.html
15. Oikeh, E. I. , Omoregie, E. S. , Oviasogie, F. E. , Oriakhi, K. . "Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates." Food Science & Nutrition 4(1): 103-109, 2015. Retrieved from: http://doi:10.1002/fsn3.268
16. Otang, W. M. , Afolayan, A. J. . "Antimicrobial and antioxidantefficacy of Citrus limon L. peel extracts used for skin diseases by Xhosa tribe of Amathole District, Eastern Cape, South Africa" South African Journal of Botany , 2016. Retrieved from: http://282411080_Antimicrobial_and_antioxidant_efficacy_of_Citrus_limon_L_peel_extracts_used_for_skin_diseases_by_Xhosa_tribe_of_Amathole_District_Eastern_Cape_South_Africa
17. UW Health(). Citric acid and kidney stones. . Retrieved August 17, 2020, from: https://www.uwhealth.org/files/uwhealth/docs/pdf/kidney_citric_acid.pdf
18. Penniston, K. L. , Nakada, S. Y. , Holmes, R. P. , Assimos, D. G. . "Quantitative assessment of citric acid in lemon juice, limejuice, and commercially-available fruit juice products." Journal of Endourology 22(3): 567-570, 2008. Retrieved from: http://doi:10.1089/end.2007.0304
19. Sekar, M. , Safwan, N. , Bin, H. , Afendi, N. , Nurul, P. , Binti, F. , Abdullah, B. . (). Comparative evaluation of antimicrobial properties of citrus varieties available in Malaysia market.. . Retrieved from: https://www.researchgate.net/publication/258048761_COMPARATIVE_EVALUATION_OF_ANTIMICROBIAL_PROPERTIES_OF_CITRUS_VARIETIES_AVAILABLE_IN_MALAYSIA_MARKET
20. Tille, P. M. . Bacterial genetics, metabolism, and structure.. Bailey & Scott’s Diagnostic Microbiology 13th Ed. St. Louis, Missouri: Elsevier, 2017. 19. . Retrieved from: https://www.nap.edu/read/23647/chapter/4#33
21. World Health Organization.(2018, February 7). Food and water borne diseases threaten 7 million Filipinos without toilet facilities. . Retrieved February 5, 2019, from: https://www.who.int/philippines/news/detail/26-01-2016-food-andwaterborne-diseases-threaten-7-million-filipinos-without-toiletfacilities#:~:text=Food%20and%20waterborne%20diseases%20threaten%207%20million%20Filipinos%20without%20toilet%20facilitieshttp:
22. Würzbach, M. , Lowin, I. , Eckel, B. . (). Effects of the storage duration on the stability of flavonoids in hoops and green tea extract. . Retrieved January 20, 2020, from: https://www.efeedlink.com/cps/attachment/2015/november/2015112615184488807350.pdf

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