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The effect of Bougainvillea spectabilis leaf and bract extract on dexamethasone - induced hyperglycemic male albino mice (Mus musculus).

Author

Ma. Carmela Batiquin,
Nathasia Rose Brizo,
Honeylou Dupa,
Leslie Marie Piñero,
Phel Angeli Pino,
Maricel  Flores

Related Institution

College of Arts and Sciences – Department of Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Biology
Publication Date
March 2019

Abstract

   Diabetes is one of the leading cause of death among Filipinos based on the data from the 2013 Philippine Health Statistics, and over 6 million Filipinos are diagnosed to have diabetes. It is a chronic disease characterized by high levels of sugar in the blood (hyperglycemia) because of inadequate production and/or action of insulin, the hormone that regulates blood sugar levels (Department of Health [DOH], 2017). Bougainvillea spectabilis is reported to have antidiabetic properties. The aim if this study is to screen potential hypoglycemic effects of Bougainvillea spectabilis leaf and bract extract. Methods: The ethanolic extract (500mg/kg) of Bougainvillea spectabilis was administered to Dexamethasone - Induced Hyperglycemic male Albino Mice. Dexamethasone is an example of glucocorticoid steroid. This drug promotes gluconeogenesis or increased blood sugar level in the blood. Blood glucose levels were measured on the 8th day after oral treatment of the said extract.


   Data was then gathered and was analyzed using the One - Way Anova Test and Bonferroni Multiple Comparisons Test. One - Way Anova revealed that post - treatment blood glucose levels (mg/dl) of the albino mice were not the same among the treatment groups at .05 level of significance; F(3,20) = 8.631,p < .05, d= .564. The effect size for this analysis (d=.564) was found to be moderate. Bonferroni post-hoc test showed that on the average, post-treatment blood glucose levels of male albino mice treated with Glibenclamide ( M=111.13, SE= 6.01) and Bougainvillea spectabilis leaf extract (M=104.13, SE=6.05) were the same. However and still on the average, post-treatment blood glucose levels of male albino mice given with distilled water (M=144.38, SE= 6.04) was significantly higher compared with those given with Glibenclamide and 100% Bougainvillea spectabilis leaf and bract extract by 33.52 mg/dl and 40.91 mg/dl, respectively. Based on the findings of Bonferroni multiple comparisons test on the 8th day post-treatment, the experimental group 100% Bougainvillea spectabilis leaf and bract extract, and positive control group, Glibenclamide, exhibited hypoglycemic effects to the Dexamethasone-induced hyperglycemic male albino mice. On the other hand, the negative control group, distilled water, did not exhibit hypoglycemic effects to the Dexamethasone-induced hyperglycemic male albino mice. The blood glucose levels between the positive control (Glibenclamide) and the Experimental (B. spectabilis Extract) showed no significant difference with each other. The Negative control (distilled water) and the Experimental (B. spectabilis Extract) showed significant difference with each other. Analyzing the results, it is clear that Bougainvillea spectabilis Leaf and Bract extract can be a good candidate for developing new neutraceuticals treatment for diabetes.


   The 100% Bougainvillea spectabilis leaf and bract extract holds potential hypoglycemic properties on the blood glucose level of Dexamethasone-induced male albino mice. The researchers would like to recommend the following: To the diabetic patients in lowering their blood glucose levels through the use of naturally prepared Bougainvillea spectabilis leaf and bract extract. To use Bougainvillea spectabilis leaf and bract extract as an alternative to expensive medication and for the teachers to offer new knowledge in treatment and control of diabetes.

References

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Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U1 Ef36ba 2019 Fulltext Print Format

 
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