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Antimicrobial potential of Zea mays linnaeus (corn) silk extract against Staphylococcus aureus ATCC no. 25923.

Author

Lauren Ashley C. Licuanan,
Melleshie Joyce A. Ares,
Ella Marie V. Ibañez,
Gabrielle Renee T. Peguit,
Aprielle Joyce L. Pelaez,
MA. LOURDES  C. UMLAS

Related Institution

College of Arts and Sciences – Department of Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Biology
Publication Date
March 2019

Abstract

   Zea mays Linnaeus (Corn) silk is an essential plant found extensively all over the world that is used traditionally to treat a wide range of diseases. Its potential use is very much related to its properties and mechanism of action of the plant. This traditional plant was found to have medicinal properties like anti-oxidant, anti-inflammatory, neuroprotective toxicity and many more properties (Vijitha & Saranya, 2017). The study is aimed mainlyat determining whether or not Zea mays Linnaeus has any antimicrobial potential by getting its silk extract and using that against the bacteria Staphylococcus aureus. Silk extracts were taken at 100% concentration and tested against S. aureus for any observable zones of inhibition that form. A Completely Randomized Design was used for this experimental study, and was conducted at the Research Laboratory of Cebu Doctor's University in Mandaue City, Cebu. The verified bacteria was obtained from Fil-Anaserve, Inc. located in Quezon City, Philippines and the plant was collected from a local source. By means of a soxhlet apparatus, silk extracts of the plant were taken. Control groups were introduced for comparison. The positive control used was Cefuroxime, which is an antibiotic known for its potency against bacteria, especially skin bacteria like S. aureus. The negative control was ethanol. Nine replicates per group (both experimental and control) were made and the Kirby Bauer Disc Diffusion Method was used for the determination of antimicrobial potential. Each experimental and control group was put in a petri dish, with a total of twenty seven (27) petri dishes used. Statistical tools were used to obtain the results. The IBM SPSS version 22 was used for statistical analysis and an alpha level of 0.05 was considered for the determination of significant differences between all groups.


   After 24 hours of incubation, the positive control (Cefuroxime), the negative control (Ethanol), and 100% concentration of the experimental group had formed observable zones of inhibition. Cefuroxime had a mean zone of inhibition of 20.62 mm with a standard deviation of 0.24 mm. Ethanol had a mean zone of inhibition of 0.05 mm with a standard deviation of 0.03 mm. The experimental group (100% concentration) had a mean zone of inhibition of 0.28 mm with a standard deviation of 0.10 mm, respectively. One-way ANOVA showed that there was a significant difference of the zones of inhibition among the groups, F (3, 24) = 12675. 713, p = 0.000. When compared with one another, the positive control (Cefuroxime) had a greater zone of inhibition.


   Based on the results it was concluded that Zea mays Linnaeus (Corn) silk extract has no antimicrobial potential against Staphylococcus aureus. Further research is needed to fully realize the potential of the plant Zea mays Linnaeus (Corn) silk.

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Cebu Doctors' University - Main Library U1 An87l 2019 Fulltext Print Format

 
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