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Antimicrobial potential of Zea mays linnaeus (corn) silk extract against Staphylococcus aureus ATCC no. 25923.


Lauren Ashley C. Licuanan,
Melleshie Joyce A. Ares,
Ella Marie V. Ibañez,
Gabrielle Renee T. Peguit,
Aprielle Joyce L. Pelaez,

Related Institution

College of Arts and Sciences – Department of Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
March 2019


   Zea mays Linnaeus (Corn) silk is an essential plant found extensively all over the world that is used traditionally to treat a wide range of diseases. Its potential use is very much related to its properties and mechanism of action of the plant. This traditional plant was found to have medicinal properties like anti-oxidant, anti-inflammatory, neuroprotective toxicity and many more properties (Vijitha & Saranya, 2017). The study is aimed mainlyat determining whether or not Zea mays Linnaeus has any antimicrobial potential by getting its silk extract and using that against the bacteria Staphylococcus aureus. Silk extracts were taken at 100% concentration and tested against S. aureus for any observable zones of inhibition that form. A Completely Randomized Design was used for this experimental study, and was conducted at the Research Laboratory of Cebu Doctor's University in Mandaue City, Cebu. The verified bacteria was obtained from Fil-Anaserve, Inc. located in Quezon City, Philippines and the plant was collected from a local source. By means of a soxhlet apparatus, silk extracts of the plant were taken. Control groups were introduced for comparison. The positive control used was Cefuroxime, which is an antibiotic known for its potency against bacteria, especially skin bacteria like S. aureus. The negative control was ethanol. Nine replicates per group (both experimental and control) were made and the Kirby Bauer Disc Diffusion Method was used for the determination of antimicrobial potential. Each experimental and control group was put in a petri dish, with a total of twenty seven (27) petri dishes used. Statistical tools were used to obtain the results. The IBM SPSS version 22 was used for statistical analysis and an alpha level of 0.05 was considered for the determination of significant differences between all groups.

   After 24 hours of incubation, the positive control (Cefuroxime), the negative control (Ethanol), and 100% concentration of the experimental group had formed observable zones of inhibition. Cefuroxime had a mean zone of inhibition of 20.62 mm with a standard deviation of 0.24 mm. Ethanol had a mean zone of inhibition of 0.05 mm with a standard deviation of 0.03 mm. The experimental group (100% concentration) had a mean zone of inhibition of 0.28 mm with a standard deviation of 0.10 mm, respectively. One-way ANOVA showed that there was a significant difference of the zones of inhibition among the groups, F (3, 24) = 12675. 713, p = 0.000. When compared with one another, the positive control (Cefuroxime) had a greater zone of inhibition.

   Based on the results it was concluded that Zea mays Linnaeus (Corn) silk extract has no antimicrobial potential against Staphylococcus aureus. Further research is needed to fully realize the potential of the plant Zea mays Linnaeus (Corn) silk.


1. Al-Sorchee, S. M. A. , Zaain, L. A. , Al Saqii, A. H. . "The effect ofnettle leaves and corn silk extracts on the isolated bacteria fromchildren UTI." Tikrit Journal for Agricultural Sciences 16(2): 2016. Retrieved from: http://https://www.iasj.net/iasj?func=fulltext&aId=110154
2. Atlas Medical.(). The antibiotic sensitivity discs.. . Retrieved November 18, 2018, from: http://atlasmedical.com/upload/productFiles/212/The%20antibiotic%20sensitivity%20discs%20(with%20dispenser)%20Package%20insert.pdf
3. Azwanida, N.N. . "A review on the extraction methods use inmedicinal plants, principle, strength and limitation." Medicinal &Aromatic Plants 4(3): 2015. Retrieved from: http://doi: 10.417212167-0412.1000196
4. Bhaigyabati, T. , Ramya, K. T. , Usha, K. . "Phytochemical constituents and antioxidant activity of various extracts of corn silk(Zea mays.L)." Research Journal of Pharmaceutical, Biological and Chemical Sciences 2(4): 986-993, 2011. Retrieved from: http://rjpbcs.com/pdf/2011_2(4)/[106].pdf
5. Cowan, M.M. . "Plant products as antimicrobial agents." Clinical Microbiology Reviews 12(4): 564-582, 1999. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/10515903
6. Docekal, J. , Hall, J. , Reese, B. , Jones, J. , Ferguson, T. . "A rare presentation of community acquired Methicillin resistant Staphylococcus aureus." Case Reports in Infectious Disease 73(Suppl): 45, 2013. Retrieved from: http://Doi: 10.1155/2013/543762
7. Elumalai, E. K. , Ramachandran, M. , Thirumalai, T. , Vinothkumar, P. . "Antibacterial activity of various leaf extracts of Merremia emarginata." Asian Pacific Journal of Tropical Biomedicine 1(5): 406-408, 2011. Retrieved from: http://doi: 10.1016IS2221-1691(11)60089-0
8. Eman, A. A. . "Evaluation of antioxidant and antibacterial activities of Egyptian Maydis stigma (Zea mays hairs) rich in some bioactive constituents." Journal of American Science 7(4): 726-729, 2011. Retrieved from: http://www.jofamericanscience.org/journals/amsci/am0704/101_5383am0704_726_729.pdf
9. Emmanuel, S. A. , Olajide, O. , Abubakar, S. , Akjode, S. O. , Etuk-Udo, G. . "Chemical evaluation, free radical scavenging activities andantimicrobial evaluation of the methanolic extracts of corn silk(Zea mays)." Journal of Advances in Medical and Pharmaceutical Sciences 9(4): 1-8, 2016. Retrieved from: http://Doi: 10.9734/JAMPS/2016/28530
10. Gupta, S. , Prabha, V. . "Human sperm interaction with Staphylococcus aureus: A molecular approach." Journal of Pathogens , 2012. Retrieved from: http://Doi: 10.1155/2012/816536
11. Hasanudin, K. , Hashim, P. , Mustafa, S. . "Corn silk (Stigmamaydis) in healthcare: A phytochemical and pharmacological review." Molecules 17(8): 9697-9715, 2012. Retrieved from: http://Doi:10.3390/molecules17089697
12. Hu, Q. L. , Zhang, L. J. , Li, Y. N. , Ding, Y. J. , Li, F. L. . "Purification and anti-fatigue activity of flavonoids from corn silk." International Journal of Physical Sciences 5(4): 321-326, 2010. Retrieved from: http://https://academicjournals.org/article/article1380726309_Hu%2520et%2520al.pdf
13. Liu, R. H. . "Health promoting compounds of fruits and vegetables inthe diet." Advances in Nutrition 4(3): 384S-392S, 2013. Retrieved from: http://doi:10.3945/an.112.003517.
14. Manandhar, S. , Luitel, S. , Dahal, R. K. . "In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria." Journal of Tropical Medicine (5): 1-5, 2019. Retrieved from: http://Doi:10.1155/2019/1895340
15. Microchem Laboratory.(). Zone of inhibition test for antimicrobialactivity.. . Retrieved January 15, 2019, from: http://microchemlab.com/test/zone-inhibition-test-antimicrobialactivity
16. Monterey Bay Spice Company.(). Zea mays corn silk cut & sifted.. . Retrieved December 18, 2018, from: http://https://www.herbco.com/p-915-cornsilk-cs.aspx
17. Morshed, S. , Islam, S.M. . "Antimicrobial activity and phytochemical properties of corn (Zea mays L.) silk ." SKUAST Journal of Research 17(1): 8-14, 2015. Retrieved from: http://https://www.researchgate.net/publication/291792510
18. Nessa, F. , Ismail , Z. , Mohamed, N. . "Antimicrobial activities of extracts and flavonoid glycosides of corn silk (Zea mays L.)." International Journal of Biotechnology for Wellness Industries 1, 115-121, 2012. Retrieved from: http://Doi: 10.6000/1927-3037/2012.02.02
19. Sahib, A. S. , Mohammed, I. H. , Hamdan, S. J. . "Use of aqueous extract of corn silk in the treatment of urinary tract infection." Journal of Intercultural Ethnopharcology 1(2): 93-96., 2012. Retrieved from: http://Doi:10.5455/jice.20120525123150
20. Sani, I. . "Application of medicinal plants to overcome antibiotic resistance in some selected multi-drug resistant clinical isolates." Research and Reviews: Journal of Pharmacognosy and Phytochemistry 2(4): 48-52, 2014. Retrieved from: http://www.rroij.com/openaccess/pdfdownload.php?download=open-access/
21. Shah, T. R. , Prasad, K. , Kumar, P. . "Maize—A potential source ofhuman nutrition and health: A review." Cogent Food & Agriculture 2(1): 2016. Retrieved from: http://Doi: 10.1080/23311932.2016.1166995.
22. Stuart , G. . (). Philippine medicinal plants: Mais.. . Retrieved September 16, 2018, from: http://www.stuartxchange.org/Mais.html
23. Stoppler, M.C. . (). Staph infection (Staphylococcus aureus).. . Retrieved September 18, 2018, from: http://https://www.medicinenet.com/staph_infection/article.htm#staph_infection_facts
24. Turnidge, J. , Rao, N. , Chang, F. Y. , Fowler, V. G. . (). Staphylococcus aureus.. . Retrieved January 18, 2019, from: http://antimicrobe.org/b237rev_2.asp?fbclid=IwAR2nPz8q8sFW7R gCf0_bEdQv7vrdQ9bTdLObDbxqkJUSNFSAActccLhE6OQ
25. Vijitha, T.P. , Saranya, D. . "Corn silk – a medicinal boon." International Journal of Chem Tech Research 10(10): 129-137, 2017. Retrieved from: http://www.sphinxsai.com/2017/ch_vol10_no10/1/(129137)V10N10CT. pdf
26. Xing, F. , Lei, W. , Tao, M. L. , Qing, Z. . "Studies on antimicrobial activity of aqueous extract of maize silk." Applied Mechanics and Materials 140, 426-430, 2012. Retrieved from: http://doi: 10.4028/www.scientific.net/AMM.140.426

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