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Larvicidal effect of Duranta erecta (linn) (golden dewdrop) leaf extracts against Aedes aegypti.


Angelica R. Arnaiz,
Axel Clainne Marie T. Gasparini,
Jiah Camille C. Manigos,
Miguel Benedict M. Ortiz,
Marie Joy T. Ronquillo,

Related Institution

College of Arts and Sciences – Department of Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
November 2018


   The study was conducted to determine the larvicidal effect of Duranta erecta (Linn) (Golden Dewdrop) leaf extract as an organic, plant-based larvicide against on 3rd to 4th instar Aedes aegypti larvae using the dilutions of 1:3, 1:1, and 100% crude extract. The positive and negative control groups were Lion Tiger Spray and ethanol, respectively. Eighteen larvae per control and experimental group and each group has three trials however, ethanol only had one trial giving a total of 288 larvae. The research utilized a completely randomized design (CRD) and was conducted at the Cebu Doctors' University Research Laboratory. Duranta erecta (Linn) (Golden Dewdrop) were collected from the researcher's garden. The leaves were removed from the stem of the plant and brought to the research laboratory for extract preparation. Two kilograms of Duranta erecta (Linn) (Golden Dewdrop) leaves were weighed, washed, and air-dried. Once they were dried, they were cut into small pieces and placed into the Soxhlet apparatus for the extraction with 99% ethanol as solvent. The solution resulting from the apparatus was placed in the rotary evaporator. The leaf extract was stored inside the bottle at room temperature. The leaf extract was diluted to obtain the necessary concentrations. Following the data collection, the data were processed and analyzed using IBM SPSS version 22.

   After exposing the larvae to each treatment condition for two hours, mortality observed among the groups were recorded. No surviving larvae were observed in each dilution of the Lion Tiger (1:3 and 1:1 dilution), while there were two dead larvae observed in the negative control (Ethanol). For the leaf extract, the study showed 40.7%, 90.7% and 100% larval mortality at dilutions of 1:3, 1:1, and 100% crude extract.

   Based on the results, the researchers concluded that there was larvicidal effect in Duranta erecta (Golden dewdrop) leaf extract against the 3rd to 4th instar Aedes aegypti larvae. The researchers recommend for larvicide manufacturers, that they will use Duranta erecta extract as a component in producing larvicides instead of harmful chemicals. Lastly for future researchers, that they will observe the effectiveness of Duranta erecta (Golden dewdrop) leaf extract on other instars of Aedes aegypti larvae and on the larvae of different mosquito species, and will use another positive control aside from Lion Tiger.


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LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U1 L329ar 2018 Fulltext Print Format

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