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Larvicidal effect of Duranta erecta (linn) (golden dewdrop) leaf extracts against Aedes aegypti.

Author

Angelica R. Arnaiz,
Axel Clainne Marie T. Gasparini,
Jiah Camille C. Manigos,
Miguel Benedict M. Ortiz,
Marie Joy T. Ronquillo,
MA. LOURDES  C. UMLAS

Related Institution

College of Arts and Sciences – Department of Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Biology
Publication Date
November 2018

Abstract

   The study was conducted to determine the larvicidal effect of Duranta erecta (Linn) (Golden Dewdrop) leaf extract as an organic, plant-based larvicide against on 3rd to 4th instar Aedes aegypti larvae using the dilutions of 1:3, 1:1, and 100% crude extract. The positive and negative control groups were Lion Tiger Spray and ethanol, respectively. Eighteen larvae per control and experimental group and each group has three trials however, ethanol only had one trial giving a total of 288 larvae. The research utilized a completely randomized design (CRD) and was conducted at the Cebu Doctors' University Research Laboratory. Duranta erecta (Linn) (Golden Dewdrop) were collected from the researcher's garden. The leaves were removed from the stem of the plant and brought to the research laboratory for extract preparation. Two kilograms of Duranta erecta (Linn) (Golden Dewdrop) leaves were weighed, washed, and air-dried. Once they were dried, they were cut into small pieces and placed into the Soxhlet apparatus for the extraction with 99% ethanol as solvent. The solution resulting from the apparatus was placed in the rotary evaporator. The leaf extract was stored inside the bottle at room temperature. The leaf extract was diluted to obtain the necessary concentrations. Following the data collection, the data were processed and analyzed using IBM SPSS version 22.


   After exposing the larvae to each treatment condition for two hours, mortality observed among the groups were recorded. No surviving larvae were observed in each dilution of the Lion Tiger (1:3 and 1:1 dilution), while there were two dead larvae observed in the negative control (Ethanol). For the leaf extract, the study showed 40.7%, 90.7% and 100% larval mortality at dilutions of 1:3, 1:1, and 100% crude extract.


   Based on the results, the researchers concluded that there was larvicidal effect in Duranta erecta (Golden dewdrop) leaf extract against the 3rd to 4th instar Aedes aegypti larvae. The researchers recommend for larvicide manufacturers, that they will use Duranta erecta extract as a component in producing larvicides instead of harmful chemicals. Lastly for future researchers, that they will observe the effectiveness of Duranta erecta (Golden dewdrop) leaf extract on other instars of Aedes aegypti larvae and on the larvae of different mosquito species, and will use another positive control aside from Lion Tiger.

References

1. Agwu, E. J. , Odo, E. G. , Ekeh, F. , Uwagbae, M. , Ngwu, G. , Ehilegbu, C. . "Bioefficacy of Duranta erecta leaf extract on yellow fever and dengue vector, Aedes aegypti Linn. in Nigeria" Journal of Medicinal Plants Research, 12(11): 124–132, 2018. Retrieved from: http://doi:10.5897/JMPR2016.6277
2. Angaye, T. C. , Ohimain, E. , SIasia, E. , Asaigne, P. , Finimio, O. . "Larvicidal activities of leaves of Niger delta mangrove plants against Anopheles gambiae" Sky Journal of Microbiology Research 2(7): 045–050, 2014. Retrieved from: http://skyjournals.org/sjmr/pdf/2014pdf/Oct/Angaye%20et%20al%20pdf.pdf?fbclid=IwAR2prXYdCI2xNkZtmnr5wn2szH1jy7wzyvuaidc7FSVxk50bg1t4DdzpXKY
3. Bar, A. , Andrew, J. . "Morphology and Morphometry of Aedes aegypti Larvae" Annual Review & Research in Biology 3(1): 1-21, 2013. Retrieved from: http://https://pdfs.semanticscholar.org/04e4/26693b12980c7cd1525ba30cb616867d916a.pdf
4. Centers for Disease Control and Prevention(). Dengue and the Aedes aegypti mosquito. . Retrieved December 12, 2017, from: http://https://www.cdc.gov/dengue/resources/30jan2012/aegyptifactsheet.pdf
5. Centers for Disease Control and Prevention(). Mosquito life-cycle. . Retrieved April 28, 2018, from: http://https://www.cdc.gov/dengue/entomologyecology/m_lifecycle.html
6. Chennaiyan, V. , Sivakami, R. , Jeyasankar, A. . "Effect of Duranta erecta Linn (Verbenaceae) leaf extracts against Army worm Spodoptera litura and Cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae)." International Journal of Advanced Research in Biological Sciences 3(3): 311-320, 2016. Retrieved from: http://ijarbs.com/pdfcopy/feb2016/ijarbs41.pdf
7. Department of Health(). Diseases and health conditions. . Retrieved May 7, 2018, from: http://https://www.doh.gov.ph/diseases-and-health-conditions
8. (). Duranta erecta. . Retrieved January 25, 2018, from: http://en.hortipedia.com/wiki/Duranta_erecta
9. Ghosh, A. , Chowdhury, N. , Chandra, G. . "Plant extracts as potential mosquito larvicides." The Indian Journal of Medical Research 135(5): 581–598, 2012. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401688/
10. Hemavathy, J. , Anitha, T. . "A study of larvicidal assay on Duranta repens Linn. and Vitex negundo Linn. against Culex quinquefasciatus say" International Journal of Advanced Science and Research 1(4): 18–20, 2016. Retrieved from: http://www.allsciencejournal.com/download/25/1-4-14.pdf
11. Jaga, L. , Dharmani, C. . "Global surveillance of DDT and DDE levels in human tissues" International Journal of Occupational Medicine and Environmental Health 16(1): 7-20, 2003. Retrieved from: http://https://www.ncbi.nlm.nih.gov/m/pubmed/12705713/?fbclid=IwAR2xqPvUa_RM2JHEKCtmCEwX2EIefhI94NJ6cEwumuKUpOEUyN7qjohX0K4
12. Jawale, C.S. . "Larvicidal activity of some saponin containing plants against the dengue vector Aedes aegypti" Trends in Biotechnology Research 3(1): 1-11, 2014. Retrieved from: http://https://pdfs.semanticscholar.org/8e8f/03ff74fd3f64ab14a822bc91109fa65bab48.pdf
13. Karamaouna, F. , Kimbaris, A. , Michaelakis, A. , Papachristos, D. , Polissiou, M. , Papatsakona, P. , Tsora, E. . "Insecticidal activity of plant essential oils against the Vine Mealybug, Planococcus ficus" Journal of Insect Science 13(142): 1-13, 2013. Retrieved from: http://doi: 10.1673/031.013.14201
14. Lee, S. C. , Kim, J. H. , Lee, S. J. . "Floating of the lobes of mosquito (Aedes togoi) larva for respiration" Scientific reports , 2017. Retrieved from: http://doi: 10.1038/srep43050.
15. Maia, M.F., , Moore, S.J. . "Plant-based insect repellents: A review of their efficacy, development and testing" Malaria Journal 10(Suppl 1): S11-S25, 2011. Retrieved from: http://doi: 10.1186/1475-2875-10-S1-S11
16. Meenakshi, S.V. , Jayaprakash, K. . "Mosquito larvicidal efficacy of the leaf extract from mangrove plant Rhizophora mucronata (Family: Rhizophoraceae) against Anopheles and Aedes species" Journal of Pharmacognosy and Phytochemistry 3(1): 78–83, 2014. Retrieved from: http://www.phytojournal.com/vol3Issue1/Issue_may_2014/19.1.pdf
17. Mondal, M. , Khalequzzaman, M. . "Toxicity of naturally occurring compounds of plant essential oil against Tribolium castaneum (Herbst)" Journal of Biological Sciences 10(1): 10-17, 2010. Retrieved from: http://https://scialert.net/fulltextmobile/?doi=jbs.2010.10.17&fbclid=IwAR0wAYjcCWG2EszNupuI0P6mP7yM0EJ2IZX3FM9Y1BVXnATtEOuaBSp336Q#370071_ja
18. Mosquito. Encyclopaedia Britannica. . Retrieved from: http://https://www.britannica.com/animal/mosquito-insect
19. (). Mosquito Species. . Retrieved December 12, 2017, from: http://https://www.rentokil.com.ph/mosquitoes/species/
20. Musau, J. K. , Mbaria, J. M , Nguta, J. M. , Mathiu, M. , Kiama, S. G. . "Phytochemical composition and larvicidal properties of plants used for mosquito control in Kwale County, Kenya" International Journal of Mosquito Research 3(3): 12–17, 2016. Retrieved from: http://www.dipterajournal.com/vol3issue3/pdf/3-1-4.1.pdf
21. Serena, M. M. , Balasubramani, M. , Rajan, K. , Gerald, I. A. J. . "Evaluation of the larvicidal activity of the leaf extracts of Duranta erecta Linn. (Verbenaceae) on the larvae of Culex quinquefascitatus (Say) (Culicidae)" Journal of Biopesticides 3(3): 582–585, 2010. Retrieved from: http://www.jbiopest.com/users/lw8/efiles/mcconnell_marie_serena_v33.pdf
22. Sharma, P. , Khandelwal, S. , Singh, T. , Vijayvergia, R. . "Phytochemical analysis and antifungal potential of Duranta erecta against some phytopathogenic fungi" International Journal of Pharmaceutical Science and Research 3(8): 2686-2689, 2012. Retrieved from: http://doi:10.13040/IJPSR.0975-8232.
23. Stuart, G.U. . (). Duranta. . Retrieved December 27, 2017, from: http://www.stuartxchange.org/Duranta?fbclid=IwAR3hJNn48oB9355i8qmMUoEhKlq7g2Ot5eail_cZxTOYgMM-_SAR9DFhrOI
24. Subsongsang, R. , Jiraungkoorskul, W. . "An updated review on phytochemical properties of “golden dewdrop” Duranta erecta" Pharmacognosy Review 10(20): 115-117, 2016. Retrieved from: http://doi:10.4103/0973–7847.194042

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U1 L329ar 2018 Fulltext Print Format

 
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