Michael Francis B. Abdon,
April May P. Berame,
Kimberlyn C. Cabatingan,
Destiny Joy A. Grandea,
Maria Lourdes C. Umlas
The study was conducted to determine the larvicidal effect of Allium cepa (Onion) extract on 3rd to 4th instar Aedes larvae using the concentrations of 1%, 10%, and 20%. The study aimed to also determine the significant difference of the percent mortality of Aedes larvae among the experimental and control groups. The positive and negative control were Baygon and distilled water respectively. Twenty five (25) larvae were replicated per group and giving a total of (900) larvae. The research utilized a completely randomized design (CRD) and was conducted at the Cebu Doctors' University Research Laboratory. Allium cepa (Onion) bulbs were bought at the local supermarket. The peels were then removed and then chopped into small pieces and a Soxhlet apparatus was used with 99% ethanol as the solvent to perform the extraction. The solvent was then evaporated with the use of a rotary evaporator. The crude extract was diluted to obtain the necessary concentrations. Following data collection, the data were processed using IBM SPSS version 22.
The study showed a percentage mortality of 0%, 7.3% and 20.7% on 1%, 10% and 20% concentrations respectively. The positive control had 100% suggesting the insecticide brand used was very effective. The Chi-square z test of proportion shows that there is a significant difference between the 10% and 20% concentrations at .05 level of significanceχ2 (1) = 11.074, p< .05. Allium cepa (Onion) bulb extract have larvicidal properties at the 10% and 20% concentrations. Overall the extract showed some potency, causing some mortality at mentioned concentrations.
Based on the findings there was larvicidal potential of Allium cepa (Onion) extract in Aedes 3rd to 4th. It is recommended to try higher concentration, check the pH levels and temperature of the water and research on the optimal water conditions for the Aedes larvae.
9. Pavithra, P. , Subhashini, A. . "Evaluation of larvicidal activity of five asteraceae plant extract against Aedesaegypti and it’s cytotoxic effect on the root tips of Allium cepa Linn." World Journal of Pharmaceutical and Life Sciences
2(5): 243-266, 2016. Retrieved from: http://wjpls.org/download/article/10092016/1476705283.pdf
11. Vinayagam, A. , Senthilkumar, N. , Umamaheswari, A. . "Larvicidal activity of some medicinal plant extracts against Malaria Vector Anopheles stephensi" Research Journal of Parasitology
3(2): 50-58, 2008. Retrieved from: http://doi:10.3923/jp.2008.50.58