i
Report an error   
Advanced Search


The hypoglycemic effect of Paspalum conjugatum (carabao grass) extract on the fasting blood sugar levels of dexamethasone- induced Mus musculus (male albino mice).

Author

Zanne I. Baritugo,
Ma. Joselle Cagas,
Christine Rebecca Ann I. Cutang,
Symple Irina C. Dabuet,
Plutarch Ioniel  Degollacion,
Danielle Riana L. Mata,
Charles Vincent  V. Medillo,
Vianneylee Napuli,
Ma. Therese Kim U. Pabuaya,
Kristle Shaine Perales,
Renelyn Keye S. Torregosa,
Aldrin Jeff  B. Autencio

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Medical Technology
Publication Date
November 2018

Abstract

       The study aimed to determine the hypoglycemic effect of Paspalum conjugatum (Carabao Grass) on the blood glucose levels of Dexamethasone Induced Mus musculus (Male Albino Mice). This is an experimental study that utilized the randomized control trial in evaluating the effects of Paspalum conjugatum (Carabao Grass) Extract on the blood glucose levels of Dexamethasone Induced Albino Mice. The study utilized twenty-four (24) healthy male albino mice weighing 19-40 grams with normal blood glucose levels within 60-130 mg/dl and with no other abnormalities. The male albino mice were allowed to acclimatize for a total of fifteen (15) days. Mice were allowed to fast for a total of eight (8) hours and baseline blood glucose levels were determined the following day. After acclimatization, the mice were injected intraperitonneally with a dosage of 11 mg/kg of dexamethasone for ten (10) days to increase the blood glucose level of more than 105 mg/dl to achieve hyperglycemia. After hyperglycemic induction with dexamethasone, the subjects were assigned randomly by using Excel into three groups, with eight (8) mice per control group: the negative control (Distilled water) group, the positive control (Glibenclamide) and the experimental control group which were treated with the methanolic Carabao grass extract. The subjects were then given treatment for ten (10) days. Blood extraction was done after treatment for ten (10) days.
       At post-treatment, the male albino mice treated with distilled water revealed a mean blood glucose level of (mg/dl) of 127.13 ± 40.72. Carabao Grass Extract (post-treatment) yielded a mean blood glucose level of 91. 88 ± 15.28 while male albino mice treated with Glibenclamide showed a mean blood glucose level of 63.13 ± 22.38. One-Way ANCOVA showed that there was a significant post-treatment mean blood glucose levels (mg/dl) of male albino mice difference among the Carabao Grass Extract, Glibenclamide, and distilled water groups at .05 level of significance, F(4,19) = 15.153, p <.05.
       Based on the findings, the methanolic extract of Paspalum conjugatum (Carabao Grass) possesses hypoglycemic property on dexamethasone induced hyperglycemic male albino mice. Based on the findings, the researchers would like to recommend promoting the use of plants as alternatives to highly expensive medications used for hyperglycemia after undergoing clinical trial. In the interest of future studies, the researchers would like to recommend conducting related studies of other plants under Poaceae family for their ability to lower blood glucose levels.

References

2. Alli Smith, Y.R. , Adanlawo, I.G. . "Hypoglycaemic effect of saponin fromthe root of Garcinia kola (Bitter Kola) on alloxan-induced diabetic rats" Journal of Drug Delivery & Therapeutics 2(6): 9-12, 2012. Retrieved from: http://doi:10.22270/jddt.v2i6.338
3. Buitrago, A. , Rojas-Vera, J. , Peñaloza, Y. . "In vitro antioxidant activityand qualitative phytochemical analysis of two Vismia (Hypericaceae)species collected in Los Andes, Venezuela." Revista De BiologíaTropical 64(4): 1431-1439., 2016. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/29465907
4. Cassi-Lit, M. . (). Bt corn & potential alternate host plants of the Asian cornborer. . Retrieved January 28, 2019, from: http://www.bic.searca.org/info_kits/btcorn_host.pdf
5. Castor, M. A. , Sumpaico, M. , Matias, R. , Geniston, L. . "Partialcharacterization of bermuda, carabao, cogon and talahib grass pollenextracts" Acta Medica Philippina 50(2): 2016. Retrieved from: http://https://www.researchgate.net/publication/320690196_Partial_characterization_of_Bermuda_Carabao_Cogon_and_Talahib_grass_pollen_extracts
6. Chifamba, O. . "The good, bad about weeds" The Herald. , January 2017. Retrieved from: http://https://www.herald.co.zw/the-goodbad-about-weeds/
7. Chimere, U. , Arome, D. , Affiong, D. , Ogechukwu, A. , Ike, C. . "Antidiabeticeffect of the methanolic leaf extract of Axonopus compressus (P.Beauv) in alloxan induced Diabetic rats" International Journal ofBiochemistry Research , 2016. Retrieved from: http://doi: 10.9734/IJBCRR/2016/25425
8. Choi, M. R. , Kwak, S. M. , Bang, S. H. , Jeong, J. , Kim, D. . "Chronicsaponin-treatment attenuates damage to the pancreas in chronic alcoholtreateddiabetic rats" Journal of Ginseng Research 41, 503-512, 2017. Retrieved from: http://doi:10.1016/j.jgr.2016.09.002
9. Choudhary, G. P. . "Free radical scavenging properties of the ethanolextract of Cynodon dactylon" Asian Journal of Plant Science and Research 5(6): 88-90, 2015. Retrieved from: http://www.imedpub.com/articles/free-radical-scavenging- properties-ofthe-ethanol-extract-of-cynodon-dactylon.pdf
10. Desai, S.D. , Desai, D.G. , Kaur, H. . "Saponins and their biological activities" Pharma Times 41(3): 13-15, 2009. Retrieved from: http://https://www.researchgate.net/publication/288126191_Saponins_and_their_biological_activities
11. Dodge, I. . (). What is the normal blood sugar of a rat/ white mouse. . Retrieved November 20, 2018, from: http://www.madsci.org/posts/archives/2001-02/982242802.Zo.r.html
12. El Barky,, A. R. , Hussein, S. A. , Alm-Eldeen, A. , Hafez, Y. , Mohamed, T. M. . "Saponins and their potential role in Diabetes Mellitus" Diabetes Management 7(1): 148-158, 2017. Retrieved from: http://https://www.openaccessjournals.com/articles/saponins-and- theirpotential-role-in-diabetes-mellitus.pdf
13. Elekofehinti, O. O. , Kamdem, J. P. , Kade, I. J. , Rocha, J. T. , Adanlawo, I. G. . "Hypoglycemic, antiperoxidative and antihyperlipidemic effects ofsaponins from Solanum anguivi Lam" fruits in alloxan-induced diabeticrats. South African Journal of Botany 88, 56-61, 2013. Retrieved from: http://doi:10.1016/j.sajb.2013.04.010
14. Evans, W.C. . Trease and Evans pharmacognosy (16th ed.) 2009. . Retrieved from: http://https://books.google.com.ph/books?id=l7pkTFyY428C&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
16. Fichna, M. , Fichna, P. . "Glucocorticoids and beta-cell function" .Endokrynologia Polska 68(5): 568-578, 2017. Retrieved from: http://doi:10.5603/EP.2017.0060
17. Freeman, V.S. . Carbohydrates. Clinical chemistry: Principles, techniques, correlations (7th ed.) 2013. 292-311. . Retrieved from: http://https://books.google.com.ph/books?id=A8-rt61zucAC&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
18. Fridlyand, L. E. , Jacobson, D.A. , Philipson, L. . "Ion channels andregulation of insulin secretion in human β-cells: A computational systemsanalysis" Islets 5(1): 1-15, 2013. Retrieved from: http://doi:10.4161/isl.24166
19. Friesen, R. . (). Burning suggested as a weed control method. . Retrieved November 21, 2018, from: http://www.manitobacooperator.ca/2009/06/11/burning-suggested-as-aweed-control-method/
21. Geer, E. B. , Islam, J. , Buettner, C. . "Mechanisms ofglucocorticoid-induced insulin resistance: Focus on adipose tissuefunction and lipid metabolism" Endocrinology and Metabolism Clinics of North America 43(1): 75-102, 2014. Retrieved from: http://doi:10.1016/j.ecl.2013.10.005
22. Geetha, T.S. , Geetha, N. . "Phytochemical screening, quantitativeanalysis of primary and secondary metabolites of Cymbopogan citratus(DC) stapf. leaves from Kodaikanal hills, Tamilnadu" InternationalJournal of PharmTech Research 6(2): 521-529, 2014. Retrieved from: http://sphinxsai.com/2014/PTVOL6/PT=17(521-529)AJ14.pdf
23. Geiser, F. . "Metabolic rate and body temperature reduction duringhibernation and daily torpor" Annual Review of Physiology 66, 239- 274, 2004. Retrieved from: http://doi: 10.1146/annurev.physiol.66.032102.115105
24. Gounarides, J.S. , Korach-André,, M. , Killary, K. , Argentieri, G. , Turner, O. , Laurent, D. . "Effect of Dexamethasone on glucose tolerance and fatmetabolism in a diet-induced obesity mouse model" Endocrinology 149(2): 758-766., 2008. Retrieved from: http://doi:https://doi.org/10.1210/en.2007-1214
25. Hari Babu, R. , Savithramma, N. . "Phytochemical screening ofunderutilized species of Poaceae" JPR:BioMedRx: An InternationalJournal 1(10): 947-951, 2013. Retrieved from: http://jprsolutions.info/newfiles/journal-file-56a4e2a7c2fc33.15159113.pdf
26. Ibeh, B. , Maxwell, E. , Bitrus, H.J. . "Phytochemical compositions and invitro antioxidant capacity of methanolic leaf extract of AxonopusCompressus (P. Beauv.)" European Journal of Medicinal Plants 3(2): 254-265, 2013. Retrieved from: http://doi:10.9734/ejmp/2013/1686
27. Jain, S. , Bhatia, G. , Barik, R. , Kumar, P. , Jain, A. , Dixit, V.K. . "Antidiabetic activity of Paspalum scrobiculatum Linn. in alloxan induceddiabetic rats" Journal of Ethnopharmacology 127(2010): 325-328, 2010. Retrieved from: http://doi:10.1016/j.jep.2009.10.038
28. Kato, A. , Miura, T. , Fukunaga, T. . "Effects of steroidal glycosides onblood glucose in normal and diabetic mice" Biological and Pharmaceutical Bulletin 18(1): 167-168, 1995. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/7735234
29. Kemertelidze, E. P. , Pkheidze, T. A. . "Tigogenin from Yucca gloriosa, Apossible raw material for the synthesis of steroid hormonal preparations" Pharmaceutical Chemistry Journal 6(12): 795-797, 1972. Retrieved from: http://doi:10.1007/BF00771391
30. Kim, J. J. , Xiao, H. , Tan, Y. , Wang, Z. , Seale, J.P. , Qu, X. . "The effectsand mechanism of saponins of Panax notoginseng on glucose metabolismin 3T3-L1 cells" The American Journal of Chinese Medicine 37(06): 1179-1189, 2009. Retrieved from: http://doi:10.1142/s0192415x09007582
31. Kuo, T. , McQueen, A. , Chen, T.C. , Wang, J.C. . Regulation of glucosehomeostasis by glucocorticoids. Glucocorticoid signaling. Advances in Experimental Medicine and Biology 2015. 99-126. . Retrieved from: http://doi:10.1007/978-1-4939-2895-8
32. Kumar, A. , Lingadurai, S. , Shrivastava, T.P. , Bhattacharya, S. , Haldar, P.K. . "Hypoglycemic activity of Erythrina variegata leaf in streptozotocininduceddiabetic rats" Pharmaceutical Biology 49(6): 577-582, 2011. Retrieved from: http://doi:10.3109/13880209.2010.529615
33. Lavle, N. , Shukla, P. , Panchal, A. . "Role of flavonoids and saponins inthe treatment of Diabetes mellitus" Journal of Pharmaceutical Scienceand Bioscientific Research 6(4): 535-541, 2016. Retrieved from: http://www.jpsbr.org/volume_6/JPSBR_Vol_6_Issue_1_htm_files/JPSBR16RS4020.pdf
34. Moses, T. , Papadopoulou, K.K. , Osbourn, A. . "Metabolic and functionaldiversity of saponins, biosynthetic intermediates and semi-syntheticderivatives" Critical Reviews in Biochemistry and Molecular Biology 49(6): 439–462, 2014. Retrieved from: http://doi.org/10.3109/10409238.2014.953628
35. Negi, J.S. , Negi, P.S. , Pant, G.J. , Rawat, M.S.M. , Negi, S.K. . "Naturally occurring saponins: Chemistry and biology" Journal of Poisonousand Medicinal Plant Research 1(1): 001-006, 2013. Retrieved from: http://apexjournal.org/jpmpr/archive/2013/May/fulltext/Negi%20et%20al.pdf
36. Niopas, I. , Daftsios, A. C. . "A validated high-performance liquidchromatographic method for the determination of glibenclamide in humanplasma and its application to pharmacokinetic studies" doi:10.1016/s0731-7085(02)00013-4 28(3-4): 653-657, 2002
37. Niu, L. , Chen, Q. , Hua, C. , Geng, Y. , Cai, L. , Tao, S. , Zhao, R. . "Effects of chronic dexamethasone administration on hyperglycemia and insulinrelease in goats" Journal of Animal Science and Biotechnology 9(1): 1-10., 2018. Retrieved from: http://doi:10.1186/s40104-018-0242-4
38. Noumi, E. , Yomi, A; . "Medicinal plants used for intestinal diseases inMbalmayo Region, Central Province, Cameroon" Fitoterapia 72, 246-254, 2001. Retrieved from: http://doi: 10.1016/S0367-326X(00)00288-4
39. Ogie-Odia, E.A. , Eseigbe, D. , Ilechie, M.N. , Erhabor, J. , Ogbebor, E. . ".Foliar epidermal and phytochemical studies of the grasses Cymbopogoncitratus (STAPF.), Axonopus compressus (P. Beauv.) and Eragrostistremula (S.W.Beauv) in Ekpoma, Edo State, Nigeria" Science WorldJournal 5(1): 20-25, 2010. Retrieved from: http://www.scienceworldjournal.org/article/view/5803
40. Papich, M. G. . Saunders handbook of veterinary drugs: Small and largeanimal(4th ed.) 2016. . Retrieved from: http://doi:10.1016/B978-0-323-24485-5.00283-7
41. Parrish, S. . (2018, March 13). How to dispose of methanol. . Retrieved August 6, 2018, from: http://https://sciencing.com/dispose-methanol-8699726.html
42. (). Paspalum conjugatum Berg. . Retrieved June 28, 2018, from: http://https://plants.jstor.org/stable/history/10.5555/al.ap.upwta.2_659
43. Poaceae. Encyclopedia Britannica 2016. . Retrieved from: http://https://www.britannica.com/plant/Poaceae
44. Rehel, S. . "Paspalum conjugatum" Herbe creole , 2013. Retrieved from: http://doi:10.2305/IUCN.UK.2011-1.RLTS.T177227A7394696.en
45. Rodriguez, J. . (). Carabao grass: Common uses and where to buy them. . Retrieved May 11, 2018, from: http://www.brownman.com/2013/07/carabao-grass.html
46. Sawe, B. E. . (2016, October 12). Native plants of the Philippines. . Retrieved March 19, 2018, from: http://https://www.worldatlas.com/articles/native-plants-ofthe-philippines.html
47. Shad, A.A. , Ahmad, S. , Ullah, R. , Abdel-Salam, N.M. , Fouad, H. , Rehman, N.U. , Hussain, H. , Saeed, W. . "Phytochemical and biologicalactivities of four wild medicinal plants" The Scientific World Journal, 2014, 1-7, 2014. Retrieved from: http://doi:10.1155/2014/857363
48. Simon, B. . "Grass phylogeny and classification: Conflict of morphology and molecules" Aliso 23(1): 259-266, 2007. Retrieved from: http://doi:10.5642/aliso.20072301.20
49. Sivasangari Ramya, S. , Vijayanand, N. , Rathinavel, S. . "Antidiabeticactivity of Cynodon Dactylon (L.) Pers. extraction in alloxan-inducedrats" International Journal of Pharmacy and Pharmaceutical Sciences, 6(4): 2014. Retrieved from: http://https://pdfs.semanticscholar.org/f4a2/366e72105444753695f37f66338142f8c228.pdf
50. Stuart, G.U. . (). Philippine medicinal plants: Laua-laua (Paspalumconjugatum P.J. Bergius) carabao grass (Liang er cao). . Retrieved March 19, 2018, from: http://www.stuartxchange.org/Laua-laua.html
51. Tayade, P.M. , Jagtap, S.A. , Borde, S. , Chandrasekar, N. , Joshi, A. . "Effect of Psoralea corylifolia on dexamethasone-induced insulin resistancein mice" Journal of King Saud University 24, 251-255, 2012. Retrieved from: http://doi:10.1016/j.jksus.2011.03.007
52. Thakur, M. , Melzig, M. F. , Fuchs, H. , Weng, A. . "Chemistry andpharmacology of saponins: Special focus on cytotoxic properties" Botanics: Targets and Therapy 1, 19-29, 2011. Retrieved from: http://doi:10.2147/BTAT.S17261

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U4 H999ba 2018 Fulltext Print Format

 
Loading…
©2022 HERDIN PLUS. All rights reserved. | Contact Us | Keep up to date