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Hypocholesterolemic effect of unripe banana ( Musa sapientum ) peel and fruit extract on lard and dexamethasone-induced dyslipidemia of male white rats (Rattus norvegicus).

Author

Guil Lawrence P. Briones,
Christine Sheila C. Butao,
Krystelle Lois S. Llamedo,
Clydeline V. Maglasang,
Jerry F. Malmis Jr.,
Mao E. Matsumoto,
Mars Alair D. Potot,
Julia Arian S. Salinguhay,
Mikella Betina L. Veloso,
Brian  M. Denney

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Medical Technology
Publication Date
November 2016

Abstract

        The study was intended to determine the hypocholesterolemic effect of banana (Musa sapientum) peel and fruit extract on lard and dexamethasone-induced dyslipidemia of male white rats. This study is a quantitative experimental research design wherein a randomized controlled trial was used. The extraction of the unripe banana peel and fruit extract was conducted at Cebu Doctors' University Research Laboratory under the supervision of a laboratory technician. The experimentation phase of the study was conducted at Cebu Doctors' University Animal Research Laboratory guided by a licensed veterinarian. Analysis of the total cholesterol was done at Prime Care Laboratories, Cebu City.
        Fifteen (15) male white rats that had no lesions and bruises, were not sickly and were not used in previous experiments were acquired for the study. The rats were acclimatized under laboratory conditions for fifteen days and were given food and water ad libitum. Baseline cholesterol levels were then determined at 60-100 mg/dl after the last day of acclimatization. After acclimatization, the rats were then fed with lard incorporated into their diets to raise cholesterol levels above 130 mg/dl. Every five days for fifteen days their cholesterol levels were measured. Lard did raise their cholesterol levels yet it was not enough to reach the optimal level of 130 mg/dl. The researchers, as guided by the veterinarian, opted to use dexamethasone, a glucocorticoid to raise the cholesterol levels. The rats were then randomly grouped into three groups: Lipitor group as the positive control and two experimental groups, unripe banana peel extract and unripe banana fruit extract. The rats were treated accordingly with the extracts and Lipitor for ten days. Based on the findings, both unripe banana peel extract (78.00±3.39 mg/dl) and unripe banana fruit extract (67.75±9.74 mg/dl) were able to lower the cholesterol levels of the male white rats in comparison with the positive control, Lipitor (82.25±16.94 mg/dl). This is attributable to the substance pectin which inhibits bile acid absorption and also by reducing cholesterol absorption (Leveille & Sauberlich, 1966). The mean cholesterol level from the three groups was computed and one-way ANOVA showed that there was no significant mean blood cholesterol level difference across treatment condition, F(2,10) = 0.676 and p > 0.05 and, F(2,10) = 1.881 and p > 0.05 on the 5th and 10th day of post-treatment respectively.
        To conclude, unripe banana fruit extract and unripe banana peel extract were both effective in lowering the cholesterol levels of the male white rats. However, the mean blood cholesterol levels of the rats obtained after the administration of the two extracts were close to the levels of the positive control, thereby giving no significant difference between the total cholesterol levels of the experimental and control group. Regardless of its ability to mimic the lowering capability of the commercial drug, both extracts caused significantly lower blood cholesterol levels in comparison to the positive control group since the unripe banana peel and fruit extract both had high amounts of cholesterol-lowering pectin.

References

1. Bananas, . (2006, November 30). The Food Journal and Food, Nutrition & Science. . Retrieved from: http://www.foodnutritionscience.com/articles/bananas/
2. Bowen, R.A. , Sethi, A. A. , Warnick, G. R. , Remaley, A. T. . Lipids and lipoproteins (Chapter 15). Clinical chemistry: Principle, techniques, and correlations (7th ed.). Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins, 2013.
3. Cheng, G. , Jiang, Y. , Chen, Y. , Yang, S. , He, S. , Liang , H. , Duan, X. . "Combined effects of pectic enzymes on the degradation of pectin polysaccharides of banana fruit." Pakistan Journal of Botany 43(3): 1501-1505, 2011. Retrieved from: http://www.pakbs.org/pjbot/PDFs/43(3)/PJB43(3)1501.pdf
4. Department of Agricu, . (2012, November 30). Banana. . Retrieved from: http://hvcc.da.gov.ph/banana.htm
5. Edenta, C. , James, D. B. , Owolabi, O. A. , Okoduwa, S. I. R. . "Hypolipidemic effects of aqueous extract of three cultivars of musa sapientum fruit peel on poloxamer-407 induced hyperlipidemic wistar rats" International Journal of Pharmaceutical Sciences and Research (IJPSR) 5(12): 1049-1054, 2014. Retrieved from: http://www.ijpsr.info/docs/IJPSR14-05-12-048.pdf
6. Gilhuly, K. . "Do bananas affect cholesterol levels?" Livestrong , January 2015. Retrieved from: http://www.livestrong.com/article/519132-do-bananas-affect-cholesterol-levels/
7. Harini, M. , Astirin, O. P. . "Blood cholesterol levels of hypercholesterolemic rat (Rattusnorvegicus) after VCO treatment." Nusantara Bioscience 1(2): 53-58, 2009. Retrieved from: http://biosains.mipa.uns.ac.id/N/N0102/N010201.pdf
8. Horigome, T. , Sakaguchi, E. , Kishimoto, C. . " Hypocholesterolaemic effect of banana (Musa sapientum L. var. Cavendishii) pulp in the rat fed on a cholesterol containing diet" The British Journal of Nutrition 68(1): 231-244, 1992. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/1327100
9. Jambora, A.A. . "Cardiovascular disease is still the country’s top killer." Inquirer.Net , December 2014. Retrieved from: http://lifestyle.inquirer.net/178609/cardiovascular-disease-is-still-the-countrys-top-killer/
10. Johns Hopkins Univer, . (). The rat. . Retrieved from: http://web.jhu.edu/animalcare/procedures/rat.html
11. Kaimal, S. , Sujatha, K. S. , George, S. . " Hypolipidaemic and antioxidant effects of fruits of Musa AAA (Checkandali) in alloxan induced diabetic rats" Indian Journal of Experimental Biology 48, 165-173, 2010. Retrieved from: http://nopr.niscair.res.in/bitstream/123456789/7342/1/IJEB%2048(2)%20165-173.pdf
12. Kiortsis, D.N. , Filippatos, T. D. , Mikhailidis, D. P. , Elisaf, M. S. , Liberopoulos, E. N. . "Statin-associated adverse effects beyond muscle and liver toxicity" Atheroscleoris 195(1): 7-16, 2007. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/17094994
13. Leveille, G.A. , Sauberlich, H. E. . " Mechanism of the cholesterol-depressing effect of pectin in the cholesterol-fed rat" The Journal of Nutrition 88(2): 209-2014, 1966. Retrieved from: http://jn.nutrition.org/content/88/2/209.extract
14. Liscum, L. . Cholesterol biosynthesis (Chapter 15).. Biochemistry of lipids, lipoproteins and membranes (4th ed.). 2002. . Retrieved from: http://bio.ijs.si/~krizaj/group/Predavanja%202011/Biochemistry%20Lipids%20Lipoproteins%20and%20Membranes/15.pdf
15. Ma, H. . "Cholesterol and human health" Nature and Science 2(4): 17-21, 2004. Retrieved from: http://www.sciencepub.net/nature/0204-supplement/04-mahongbao.pdf
16. Marshall, T. . "New insights into the statin-cholesterol controversy" Journal of American Physicians and Surgeons 19(2): 42-46, 2014. Retrieved from: http://www.jpands.org/vol19no2/marshall.pdf
17. Maurya, D. , Belgamwar, V. , Tekade, A. . "Microwave induced solubility enhancement of poorly water soluble atorvastatin calcium." The Journal of Pharmacy and Pharmacology 62(11): 1599-1606, 2010. Retrieved from: http://doi: 10.1111/j.2042-7158.2010.01187.x.
18. Morton, J. . Banana (pp.19-46). Fruits of warm climate 1987. . Retrieved from: http://hort.purdue.edu/newcrop/morton/banana.html
19. Nordqvist, J. . " Atherosclerosis: Causes, symptoms and treatments." MNT , August 2015. Retrieved from: http://www.medicalnewstoday.com/articles/247837.php
20. Putra, N. . "Lowering cholesterol using banana skins" The Jakarta Post , September 2014. Retrieved from: http://www.thejakartapost.com/news/2014/09/17/lowering-cholesterol-using-banana-skins.html
21. Safaei, N. , Shomali, T. , Taherianfard, M. . "Niacin ameliorates lipid disturbances due to glucocorticoid administration in rats" Iranian Journal of Basic Medical Sciences 15(4): 997-1002, 2012. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586902/
22. Sampath Kumar, K.P. , Bhowmik, D. , Duraivel, S. , Umadevi, M. . "Traditional and medical uses of banana" Journal of Pharmacognosy and Phytochemistry 1(3): 51-63, 2012. Retrieved from: http://www.phytojournal.com/vol1Issue3/Issue_sept_2012/9.1.pdf
23. (). Side effects of cholesterol-lowering statin drugs. WebMD. . Retrieved from: http://www.webmd.com/cholesterol-management/side-effects-of-statin-drugs#2
24. The George Mateljan , . (). Bananas. . Retrieved from: http://www.whfoods.com/genpage.php?tname=foodspice&dbid=7
25. The Rat Fan Club, . (2013, November 30). Normal lab values. . Retrieved from: http://www.ratfanclub.org/values.html
26. Verma, C. , R.K., S. , Singh, R. B. , Mishra, S. . " Biochemical and in-silico studies on pectin methylesterase from G9 variety of Musa acuminata for delayed ripening." The Open Biochemistry Journal 9, 15-23, 2015. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407003/
27. Vila, A. C. . " Everything you need to know about cholesterol before it’s too late." The Philippine Star. , September 2014. Retrieved from: http://www.philstar.com/health-and-family/2014/09/30/1374902/everything-you-need-know-about-cholesterol-its-too-late
28. von Loesecke, H. . "The banana – A challenge to chemical investigation" Journal of Chemical Education 7(7): 1537, 1930. Retrieved from: http://pubs.acs.org/doi/pdf/10.1021/ed007p1537
29. World Health Organiz, . (). Global Health Observatory (GHO) data: Raised cholesterol.. . Retrieved from: http://www.who.int/gho/ncd/risk_factors/cholesterol_text/en/

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U4 H999b 2016 Fulltext Print Format

 
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