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Hypoglycemic effect of Swietenia macrophylla (mahogany) fruit seed extract on dexamethasone-induced hyperglycemic Mus musculus (albino mice).

Author

Fe Faye Centillas,
Emily Nicole Chamen,
Demi Louise Del Mar,
Cesar Misador Jr.,
Reevah Charisse Pepito,
Aldrin Jeff  B. Autencio

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Medical Technology
Publication Date
December 2017

Abstract

        This study aimed to determine the hypoglycemic effect of Mahogany Fruit Seed Extract (Swietenia macrophylla) on the blood glucose levels of Dexamethasone-Induced Albino Mice (Mus musculus). This is an experimental study that utilized the randomized controlled trial in evaluating the effects of Mahogany Fruit Seed Extract (Swietenia macrophylla) on the blood glucose levels of Dexamethasone-induced albino mice. The study utilized sixteen (16) healthy male albino mice weighing 19-29 grams with normal blood glucose levels within 60-130mg/dl and with no other abnormalities. The albino mice were acclimatized for a period of fourteen (14) days and baseline glucose levels were determined on the eighth & fifteenth day. After the acclimatization period, the mice were injected intramuscularly with a dosage of1 mg/kg of Dexamethasone for seven (7) days to increase the blood glucose level of more than 130 mg/dL to achieve hyperglycemia. After hyperglycemic induction with dexamethasone, the subjects were assigned randomly by using Excel into two groups, with eight (8) mice per group: the positive control (Glibenclamide) group and the experimental group of 100% concentration of Mahogany fruit seed extract. The subjects were given treatment for fifteen (15) days. Blood extraction were done on the 8th and 15th day of treatment.
        The albino mice treated with 100% Mahogany Fruit Seed Extract revealed a mean blood glucose level of (mg/dl) of 130.14 ± 9.74 on the 8th day and 103.86 ± 17.39 on the 15th day. While the Glibenclamide (positive control) group showed a mean blood glucose level of 133.25 ± 9.22 on the 8th day and 115.25 ± 14.44 on the 15th day. Independent t-test revealed that there was no significant difference in the blood glucose levels (mg/dl) among the Mahogany Fruit SeedExtract and Glibenclamide group at 0.05 level of significance, t(13) = -.634 and p > .05. Likewise, Independent t-test revealed that 15th day post treatment blood glucose levels (mg/dL) of the white albino mice in the mahogany fruit seed extract (Mean = 103.86) and glibenclamide (Mean = 115.25) treatment groups had the same blood glucose levels at 15th day post-treatment.
        Based on the study, the 100% Mahogany (Swietenia macrophylla) fruit seed extract possesses hypoglycemic properties on the blood glucose of dexamethasone-induced hyperglycemic albino mice.

References

1. Amarasuriyan, C. , Raju, K. , Raja, A. . " Phyto-chemical studies and in vitro free radical scavenging activity of Swietenia mahagoni (L.) Jacq." Indian Journal of Natural Sciences 4(23): 2014. Retrieved from: http://www.tnsroindia.org.in/JOURNAL/ISSUE%2023%20.pdf
2. Azeez, O. H. , Kheder, A. E. . " Effect of Gundelia tournefortii on some biochemical parameters in dexamethasone-induced hyperglycemic and hyperlipidemic mice." Iraqi Journal of Veterinary Sciences 26(2): 73-79, 2012. Retrieved from: http://www.vetmedmosul.org/ijvs/media/12-2-4e.pdf
3. Babar, B. D. , Estaño, I. J. P. , Go, M. G. , Orozco, A. L. , Pajugot, N. V. O. , Solabo, M. E. S. , Yu, S. M. L. . The effect of Cebu grown aloe vera gel (Aloe barbadensis miller) and commercially processed aloe gel on the blood glucose levels of albino mice (Mus musculus) . Mandaue City. 2013. Cebu Doctors’ University,
4. Babu, P. V. A. , Liu, D. , Gilert, D. R. . "Recent advances in understanding the anti-diabetic actions of dietary flavonoids" Journal of Nutritional Biochemistry 24(11): 2013. Retrieved from: http://doi: 10.1016/j.jnutbio.2013.06.003
5. Bartnik, M. , Facey, P. C. . (2016, November 30). Pharmacognosy. . . Retrieved from: https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/saponin
6. Bernal-Mizrachi, C. , Weng, S. , Feng, C. , Finck, B. N. , Knutsen, R. H. , Leone, T. C. , Semenkovich, C. F. . "Dexamethasone induction of hypertension and diabetes is PPAR-alpha dependent in LDL receptor–null mice" Nature Medicine 9(8): 1069-1075, 2003. Retrieved from: http://doi: 10.1038/nm898
7. Candasamy, M. , Murthy, T. E. G. K , Gubiyappa, K. S. , Chellappan, D. K. , Gupta, G. . "Alteration of glucose lowering effect of glibenclamide on single and multiple treatments with fenofibrate in experimental rats and rabbit models" Journal of Basic and Clinical Pharmacy 5(3): 62-67, 2014. Retrieved from: http://doi:10.4103/0976-0105.139728
8. De, D. , Chatterjee, K. , Ali, K. M. , Bera, T. K. , Ghosh, D. . "Antidiabetic potentiality of the aqueous-methanolic extract of seed of Swietenia mahagoni (L.) Jacq. in streptozotocin-induced diabetic male albino rat: A correlative and evidence-based approach with antioxidative and antihyperlipidemic activities" Evidence-Based Complementary and Alternative Medicine 2011, 2011. Retrieved from: http://doi:10.1155/2011/892807
9. (2009, November 30). Dexamethasone. . Retrieved from: https://www.rxlist.com/dexamethasone-drug/patient-images-side-effects.htm#missdose
10. (). Dexamethasone side effects. . Retrieved from: https://www.drugs.com/sfx/dexamethasone-side-effects.html
11. Dodge, I. . (2000, November 30). Re: Message . . Retrieved from: http://www.madsci.org/posts/archives/2001-02/982242802.Zo.r.html
12. Durai, M. V. , Balamuniappan, G. , Anandalakshmi, R. , Geetha, S. , Senthil Kumar, N. . "Qualitative and quantitative analysis of phytochemicals in crude extract of big – leaf mahogany (Swietenia macrophylla King)." International Journal of Herbal Medicine 4(6): 88-91, 2016. Retrieved from: http://www.florajournal.com/archives/2016/vol4issue6/PartB/4-4-6-923.pdf
13. Durstine, J. L. , Gordon, B. , Wang, Z. , Luo, X. . "Chronic disease and the link to physical activity" Journal of Sport and Health Science 2(1): 2013. Retrieved from: http://doi: 10.1016/j.jshs.2012.07009
14. Eid, A. M. M. , Elmarzugi, N. A. , El-Enshasy, H. A. . "A review on the phytopharmacological effect of Swietenia macrophylla" International Journal of Pharmacy and Pharmaceutical Sciences 5(Suppl.3): 2013. Retrieved from: http://https://www.researchgate.net/publication/257929765_A_review_on_the_phytopharmacological_effect_of_Swietenia_macrophylla
15. El Barky, A. R. , Hussein, S. A. , Alm-Eldeen, A. A. E. , Hafez, Y. A. , Mohamed, T. M. . " Saponins and their potential role in diabetes milletus." Diabetes Management 7(1): 2017. Retrieved from: http://www.openaccessjournals.com/articles/saponins-and-their-potential-role-in-diabetes-mellitus.pdf
16. Falah, S. , Safithri, M. , Katayama, T. , Suzuki, T. . " Hypoglycemic effect of mahogany (Swietenia macrophylla King) bark extracts in alloxan – induced diabetic rats." Wood Research Journal 1(2): 89-94, 2010. Retrieved from: http://repository.ipb.ac.id/bitstream/handle/123456789/76056/ART2010%20sfa.pdf?sequence=1&isAllowed=y
17. Fowler, M. J. . " Microvascular and macrovascular complications of diabetes" Clinical Diabetes 26(2): 77-82, 2008. Retrieved from: http://doi: 10.2337/diaclin.26.2.77
18. Fox, J. G. , Barthold, S. W. , Davisson, M. T. , Newcomer, C. E. , Quimby, F. W. , Smith, A. L. (Eds.). . The mouse in biomedical research (2nd ed., vol. 3). 2007. . Retrieved from: http://https://books.google.com.ph/books?id=Gi6rIvx7Ni4C&printsec=frontcover&dq=the+mouse+in+biomedical+research&hl=en&sa=X&ved=0ahUKEwi72Y3a4bbaAhVGO7wKHQ0hDBoQ6AEIJjAA#v=onepage&q=the%20mouse%20in%20biomedical%20research&f=false
19. (). Glibenclamide: Phamacology. . Retrieved from: https://www.infomed.ch/100drugs/glibphar.html
20. Hashim, M. A. , Yam, M. F. , Hor, S. Y. , Lim, C. P. P. , Asmawi, M. Z. , Sadikun, A. . "Anti-hyperglycaemic activity of Swietenia macrophylla king (meliaceae) seed extracts in normoglycaemic rats undergoing glucose tolerance tests." Chinese Medicine , 2013. Retrieved from: http://doi: 10.1186/1749-8546-8-11
21. Go, M. R. . (2016, November 30). Green stevia: Support the fignt against diabetes. . . Retrieved from: http://taste.company/taste/healthy-living/green-stevia/
22. Jensen, T. L. , Kiersgaard, M. K. , Sorensen, D. B. , Mikkelsen, L. F. . "Fasting of mice: A review" Laboratory Animals 47(4): 2013. Retrieved from: http://doi: 10.1177/0023677213501659
23. Joseph, B. , Jini, D. . "Antidiabetic effects of Momordicacharantia (bitter melon) and its medicinal potency" Asian Pacific Journal of Tropical Disease 3(2): 93-102, 2013. Retrieved from: http://10.1016/S2222-1808(13)60052-3
24. Kalaivanan, K. , Pugalendi, K. V. . "Antihyperglycemic effect of the alcoholic seed extract of Swietenia macrophylla on streptozotocin-diabetic rats." Pharmacognosy Research 3(1): 67-71, 2011. Retrieved from: http://doi:10.4103/0974-8490.79119
25. (2013, November 30). Re: Mahogany seeds for health benefits and side effects. . Retrieved from: https://hannairthy.weebly.com/home-remedies/category/all
26. Majumdar, M. . (2013, November 30). Health benefits of flavonoids.. . Retrieved from: https://www.medindia.net/patients/lifestyleandwellness/health-benefits-of-flavonoids.htm
27. Modak, M. , Dixit, P. , Londhe, J. , Ghaskadbi, S. , Devasagayam, T. P. A. . "Indian herbs and herbal drugs used for the treatment of diabetes." Journal of Clinical Biochemistry and Nutrition 40, 163-173, 2007. Retrieved from: http://doi: 10.3164/jcbn.40.163
28. Moghadamtousi, S. Z. , Goh, B. H. , Chan, C. K. , Shabab, T. , Kadir, H. A. . "Biological activities and phytochemicals of Swietenia macrophylla King. " Molecules 18(9): 10465-1048, 2013. Retrieved from: http://doi:10.3390/molecules180910465
29. Mursiti, S. , Matsjeh, S. , Jumina, , Mustofa, . "Hypoglycemia effect of saponin from oil-free mahogany seed (Swietenia macrophylla, King)" International Conference on Mathematics, Science and Education., 2014. . Retrieved from: http://icmseunnes.com/2015/wp-content/uploads/2015/10/C11.pdf
30. Nadi, F. T. Z. . Phytochemical screening, membrane stabilizing activity and cytotoxicity of ethanol extract of Swietenia mahagony leaf . 2015. BRAC University, . Retrieved from: http://dspace.bracu.ac.bd/jspui/bitstream/10361/4662/1/Nadi%20Zohora.pdf
31. Orwa, C. , Mutua, A. , Kindt, R. , Jamnadass , R. , Anthony, S. . (2008, November 30). Agroforestree database: A tree species reference and selection guide version 4.0. . Retrieved from: http://www.worldagroforestry.org/treedb/AFTPDFS/Swietenia_macrophylla.PDF
32. Patwardhan, B. , Vaidya, A. D. B. , Chorghade, M. . "Ayurveda and natural products drug discovery" Current Science 86(6): 789-799, 2004. Retrieved from: http://https://pdfs.semanticscholar.org/8925/b09bf48e0f76d30a7dc1ddd95013e21b9c8c.pdf
33. Phanse, M. A. , Patil, M. J. , Abbulu, K. . "Synthesis, characterization and evaluation of the suppression of insulin resistance in Type-II diabetes mellitus animals by treatment with metal complex" Saudi Journal of Biological Sciences 23, 420-425, 2016. Retrieved from: http://doi: 10.1016/j.sjbs.2015.08.017
34. Rai, A. , Eapen, C. , Prasanth, V. G. . " Interaction of herbs and glibenclamide: A review" International Scholar Research Network Pharmacology 2012, 2012. Retrieved from: http://doi: 10.5402/2012/659478
35. Rico, . (2009, November 30). Re: Government reforestation efforts on mahogany plantations: Pushing our native trees toward extinction. . Retrieved from: http://philippinenativeforesttrees.blogspot.com/2010/02/government-reforestation-efforts-on.html
36. Ruohonen, S. T. , Ranta-Panula, V. , Bastman, S. , Chrusciel, P. , Scheinin, M. , Streng, T. . "Potentiation of glibenclamide hypoglycaemia in mice by MK-467, a peripherally acting alpha2-adrenoceptor antagonist." Basic & Clinical Pharmacology & Toxicology 117, 392-398, 2015. Retrieved from: http://doi: 10.1111/bcpt.12440
37. Shah, M. D. , Hossain, M. A. . " Total flavonoids content and biochemical screening of the leaves of tropical endemic medicinal plant Merremia borneensis." Arabian Journal of Chemistry 7, 1034-1038, 2014. Retrieved from: http://doi: 10.1016/j.arabjc.2010.12.033
38. Shetty, A. J. , Choudhury, D. , Rejeesh, , Nair, V. , Kuruvilla, M. , Kotian, S. . " Effect of the insulin plant (Costusigneus) leaves on dexamethasone-induced hyperglycemia." International Journal of Ayurveda Research 1(2): 100-102, 2010. Retrieved from: http://doi: 10.410/0974-7788.64396
39. Smith, Y. R. A. , Adanlawo, I. G. . "Hypoglycaemic effect of saponin from the root of garcinia kola (Bitter kola) on alloxan-induced diabetic rats." Journal of Drug Delivery amd Therapeutics 2(6): 2012. Retrieved from: http://jddtonline.info/index.php/jddt/article/view/338/181
40. Sukirmansyah , . (2016, November 30). Re: Benefits and side effects of mahogany . . Retrieved from: https://steemit.com/blog/@sukirmansyah/benefits-and-side-effects-of-mahogany-2017119t23642110z
41. Swami, S. B. , Thakor, N. J. , Haldankar, P. M. , Kalse, S. B. . " Jackfruit and its many functional components as related to human health: A review." Comprehensive Reviews in Food Science and Food Safety 11(6): 565- 576, 2012. Retrieved from: http://doi:10.1111/j.1541-4337.2012.00210.x
42. Tappy, L. , Randin, D. , Vollenweider, P. , Vollenweider, L. , Scherrer, U. , Jequier, E. . "Mechanisms of dexamethasone-induced insulin resistance in healthy humans." The Journal of Clinical Endocrinology & Metabolism 79(4): 1063-1069, 1994. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/7962275
43. Wild, S. , Roglic, G. , Green, A. , Sicree, R. , King, H. . "Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030" Diabetes Care 27(5): 2004. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/15111519
44. Yao, H. , Xu, W. , Shi, X. , Zhang, Z. . "Dietary flavonoids as cancer prevention agents" Journal of environmental Science and Health 29(1): . Retrieved from: http://doi: 10.1080/10590501.2011.551317
45. Yeh, G. Y. , Eisenberg, D. M. , Davis, R. B. , Phillips, R. S. . "Use of complementary and alternative medicine among persons with diabetes mellitus: Results of a national survey." American Journal of Public Health 92(10): 1648- 165, 2002. Retrieved from: http://doi:10.2105/ajph.92.10.1648

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