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Larvicidal potential of Hibiscus rosa-sinensis (China Rose) leaf extract against Aedes mosquito larvae.


Rekha Choudhary,
Murali Krishna Digumarthy,
Anishvi Gudapati,
Tejaswini Sarvamthota ,
Indu Malothu,
Desiderio C. Asane

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
May 2017


This study was conducted to determine the larvicidal potential of Hibiscus rosa-sinensis leaf extract on the Aedes mosquito larvae. A completely randomized design was used in the present study. Concentrations of 10%, 25%, 45% of Hibiscus rosa-sinensis leaf extract were prepared and used to determine their larvicidal potential. The commercial larvicide Baygon was used as positive control and distilled water as negative control and three different concentrations of plant extract to represent a total of five(5) treatment groups. The leaves of Hibiscus rosa-sinensis were air dried for a couple of weeks and powdered using an electric blender and the powder was gone through the extraction process using Soxhlet apparatus and rotary evaporator. The crude extract was filtered by using filter papers and solid impurities were separated. Ethanol was used as solvent for the extraction process. A total of 14 replicates for each concentration including the control groups were used resulting to a total of 70 replicates. Each replicate was treated with Five (5) Aedes mosquito larvae and were exposed to the treatment groups and were observed after one hour period. All data taken during experimentation were recorded and tabulated.

Among the treatment groups, Baygon as the positive control showed the maximum effect with 100% mortality while the negative control did not show any mortality. The experimental group concentration of 25% and 45% showed a significant effect but less compared to the 10% concentration. There was a significant difference in the larval mortality (%) among the treatment groups based on Kruskal Wallis test, H (2) = 7.358, pThe test revealed that 10% concentration group (Median = 10%) had the same percent larval mortality with the 25% concentration group (Median = 20%). The 10% concentration group had significantly smaller larval mortality compared with 45% concentration group. It should be noted that 25% concentration group had the same percent larval mortality with the 45% concentration group.

As we noted the death of Aedes larvae it is therefore concluded that the leaf extract of Hibiscus rosa-sinensis had larvicidal potential. In the interest of future studies, the researchers would like to recommend the following: a higher concentration of extracts could be used like 75%, the period of exposure can be increased from hours to days.


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