Report an error   
Advanced Search

Antibacterial potential of Theobroma cacao linn (Cacao) crude leaf extract against Staphylococcus aureus.


Pranav Varshith Ittadi,
Tirumalarao Maguluri,
Samreen Shaik,
Rama Lakshmi Marpalligolla,
Nitheesh Raja Mallavarapu,
Elvira A. Arong

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
June 2017


          Theobroma  cacao (cacao)  is  a  popularly  known  as  chocolate tree  and  its  contain  a  high  nutritional  value and  its  medical  properties.  Cacao  used  as  a  flok  medicine (tribal)  and  its mainly  grows  in  near  seaways  because  they  require  a  high  humidity  for  their  growth.  Cacao used  as  a  medicine  for  so  many  diseases. The  researchers  of  this  study  had  mainly  aimed  to  find  the  effect  of  cacao  leaf  extracts  on  the  growth  of  the  most  common  bacteria that  causes  various  skin  infections.  Staphylococcus  aureus  is  one  of  the  most  common  bacteria,  its  effect  the  70%  of  the  human  population. Specifically, this  research  used  the  Cacao  leaves at  50,75  and  90  milliliter  per  disc  concentration  in  order  to  determine  the  said  potential.  The  experimental  study  used  a  Completely  Randomized  Design.  Randomization  was  done  by  obtaining  random  colonies  from  a  culture  of  staphylococcus  aureus obtained  from  Fil-Ana serve, Inc. 3rd flr, MRL Tower 124 Malakas St. Central Diliman Quezon City.  Cacao  leaves  were  collected  at   Mandaue  City,  Cebu. 

           The  highest  mean  zone  of  inhibition  of  the  cacao  leaf  extract  is  at  90%  concentration.  As  per  the  results  as  the  concentration  increased,  its  effectivity  also  increased.  The  zone  of  inhibition  observed  within  the  experimental  group  was  due  to  the  presence  of  tannis,  flavonoids  and  some  other  secondary  metabolites. A nonparametric Kruskal-Walls test revealed that there was a significant median zone of inhibition difference among the experimental and control groups at 0.05 level of significance. H(3)= 12.049, p

          The  leaf  extracts  of  the  Theobroma  cacao  had  shown  an  antimicrobial  potential  against  the  bacteria  thus  the  presence  of  tannis,  flavonoids  like  secondary  metabolites  in  the  leaves  that  function  as  antimicrobials  had  been  confirmed.  It  was  concluded  that  although  the  different  extract  exhibited  a  zone  of  inhibition,  it  can be  used  as a  substitute  for  antibiotics  that  are commercially  available  in  pharmacies      or     drugstores    because     its     level     of      effectivity. 


1.  Biologicalclassific, . (). . Retrieved from: http://ncert.nic.in/ncerts/l/kebo102.pdf
2. Chao, S. , Young, G. , Oberg, C. , Nakaoka, K. . "Inhibition of methicillin resistant staphylococcusaureus by essential oils" Flavour andFlagrance Journal 23(6): 444-449, . Retrieved from: http://onlinelibrary.wiley.com/doi/10.1002/ffj.1904/abstract
3. Chuah, E.L , Zakaria, Z.A , Suhali, Z. , Abu Bakar, S. , Desa, M.N . "Antimicrobial  activities of  plant  extracts against  methicillinsusceptible  and  methicillin-resistant  Staphylococcus  aureus" Journal  of Microbiology  Research 4(1): 6-13, . Retrieved from: http://doi:  10.5923/j.microbiology.  20140401.02
4. Thediscovery and nam, . (). . Retrieved from: http://www.antimicrobe.org/h04c.files/history/S-aureus.pdf
5. Scapagnini, G. , Davinelli, S. , Di Renzo, L. , De Lorenzo, A. , Olarte, H.H , Micali, G. , Gonzales, S. . "Cocoa bioactive compounds: significance and potential for the maintenance of skin health" Nutrients 6, 3202-3213, . Retrieved from: http://doi: 10.3390/nu6083202
6. Harris, L.G , Foster, S.J , Richards, R.G . "An introduction to Staphylococcus aureus, and techniquesfor identifying and quantifying s.aureus adhesions in relation to adhesion tobiomaterials: Review" European Cells andMaterials 4, 39-60, . Retrieved from: http://www.ecmjournal.org/journal/papers/vol004/pdf/v004a04.pdf
7. Kowti, R. , Harsha, R. , Mohammed, G. A., Hareesh, A.R , Thammanna Gowda, S.S , Dinesha, R. , Ali, M. . "Antimicrobial activity of ethanol extract of leaf and flower of Spathodea camoanulata P.Beauv" Research Journal of Pharmaceutical, Biological and Chemical Sciences 1(3): 691-692, . Retrieved from: http://www.rjpbcs.com/pdf/2010_1(3)/80.pdf
8. Mahesh, B. , Satish, S. . " Antimicrobial activity of some important Medicinal plant against plant and human pathogens" WorldJournal ofAgricultural Sciences 4, 839-843, . Retrieved from: http://www.idosi.org/wjas/wjas4(s)/7.pdf
9. Panganiban, C.A , Reyes, R.B , Agojo, I. , Armedilla, R. , Consul, J. , Dagli, H. , Esteban, L. . "Antibacterial  activity  of cacao(TheoBroma  Cacao  Linn.) pulp  crude  extracts against  selected  isolates" NationalPeer Reviewed Journal 4, 169-181, . Retrieved from: http://www.ijppsjournal.com/vol4Issue2/3545.pdf
10. Samak, G. , Ballal, M. , Devadiga, S. , Shenoy, R. , Vinayaka, K.S . "Inhibition of methicillin resistantStaphylococcus Aureus by  botanicalextracts of wagatea spicata" International  Journal of Pharmacy and PharmaceuticalSciences 4(2): . Retrieved from: http://www.ijppsjournal.com/Vol4Issue2/3545.pdf
11. Santos, R.X , Oliveria, G.A , Sodre, G.A , Gosmann, G. , Brendel, M. , Pungartnik, C. . "Antimicrobialactivity of fermented Theobroma cacaopod husk extract" Genetics and MolecularResearch 13(3): 7725-7735, . Retrieved from: http://www.funpecrp.com.br/gmr/year2014/vol13-3/pdf/gmr3759.pdf
12. Zainal, B. , Abdah, M.A , Taufiq, Y.H , Roslida, A.H , Rosmin, K. . "Anticancer  agents from  non-edible  parts of  Theobroma  cacao" NaturalProducts Chemistry and Research 2(134): . Retrieved from: http://doi: 10.4172/2329-6836.1000134

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors U1 An87i 2017 Fulltext Print Format

©2022 HERDIN PLUS. All rights reserved. | Contact Us | Keep up to date