Report an error   
Advanced Search

The insecticidal effect of acetogenin extract from Annona muricata (Guyabano) seeds on Blatta lateralis (Turkestan cockroaches).


Faith R. Agbuya,
Ann Murry R. Dinglasa,
Tiffani Amber Q. Montebon,
April Diane L. Ruiz,
Decyril Mae M. Tayaban,
Kevin Christian Dela Cruz

Related Institution

College of Pharmacy - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
March 2017


This study entitled "The Insecticidal effect of Acetogenin extract from Annona muricata (Guyabano) seeds on Blatta lateralis (Turkestan cockroaches)" sought to determine the insecticidal effect of the Acetogenin extract of Annona muricata seeds on Turkestan cockroaches using a post-test only control group design. The study consisted of 135 verified Turkestan cockroaches which were randomly divided into three groups, where each group had 45 cockroaches and were further divided into three trials. The study was conducted in room 423, the Pharmaceutical Laboratory of Cebu Doctors' University. After the acclimatization of the test subjects for fourteen days at normal room temperature, the three groups were then treated with the following solutions: The positive control group was sprayed three times with Propoxur (Baygon); the Experimental group was sprayed 3 times with Acetogenin extract, which was obtained after simultaneous maceration from three solvents (hexane, ethyl acetate, and ethyl alcohol); and the Negative control group was sprayed 3 times with distilled water.

The Percent Mortality of Turkestan cockroaches among the three groups four hours after treatment varied. It showed that the Positive control group and the Negative control group resulted to the expected mortality (%) of 100% and 0% respectively while the Experimental group resulted in mortality (%) of 71.1%. The results showed that Propoxur (Bayggon) had the highest mortality (%) having all cockroaches dead, whereas the negative group, which distilled water was used, resulted in no number of deaths. The result of the Experimental group mortality (%) implicates that not all cockroaches died. Although the mortality (%) of the Experimental group showed a slight distinction with the Positive control group, the Acetogenin extract was regarded to have an insecticidal effect since above 50% of percent insecticidal activity is considered effective. Also, it is evident that the mortality (%) of the Experimental treatment is of great distinction as to the Negative control group which has no insecticidal effect at all. The mortality (%) after treatment were analyzed by Chi-Square test of proportion which revealed that overall; there was a significant difference in the proportion across the groups with a p-value of 0.000 to the three groups at a 0.05 alpha level of significance.

Therefore, the insecticidal effect of Acetogenin extract from Annona muricata (Guyabano) seeds is proven to be effective against Blatta lateralis (Turkestan cockroaches). The researchers would like to recommend future researchers to conduct further study on the toxicity of Acetogenin extract and its effect on other specific insects.


1. (2015, November 30). Annonacin datasheet. . Retrieved from: http://www.chemfaces.com/manual/Annonacin-CFN97856.pdf
2. (2016, November 30). Blatta lateralis. Integrated Taxonomic Information System (ITIS). . Retrieved from: https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN &search_value=666676#null
4. Cecchine, G. , Golomb, B. A. , Hilborne, L. H. , Spektor, D. M. , Anthony, C.R. . (1999, November 30). A review of the Scientific literature as it pertains to Gulf War illnesses (Vol.8). . Retrieved from: http://www.gulflink.osd.mil/library/randrep/pesticides_paper/
5. DeAngelis, J. . (2015, November 30). Natural pesticides.. . Retrieved from: www.livingwithbugs.com/natural_pesticides.html
7. Feldman, J. (Ed.) . "Chemical watch factsheet: Propoxur" Pesticides and you 30(4): 21-23, 2011. Retrieved from: http://www.beyondpesticides.org/assets/media/documents/infoservices/p esticidesandyou/documents/Winter10-11vol.30no.4.pdf
8. Gilbert, S. . (2013, November 30). Pesticides. . Retrieved from: http://www.toxipedia.org/display/toxipedia/pesticides
9. Gupta, A. , Pandey, S. , Shah, D.R , Yadav, J.S. , Seth, N.R. . "Annonaceous Acetogenins: The Unrevealed Area for Cytotoxic and Pesticidal Activities. " Systematic Reviews in Pharmacy 2(2): 104-109, 2011. Retrieved from: http://www.sysrevpharm.org/sites/default/files/2-7_0.pdf
10. Hahn, J. , Ascerno, M. E. . (2015, November 30). Cockroches. . Retrieved from: https://www.extension.umn.edu/ garden/insects/find/cockroaches/
11. Isman, M. B. . "Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world." Annual Reviews Entomololgy 51, 45-66, 2006. Retrieved from: http://doi:10.1146/annurev.ento.51.110104.151146
12. Isman, M. B. , Seffrin, R. . "Natural insecticides from the Annonaceae: A unique example for developing Biopesticides." Advances in Plant Biopesticides , 2014. Retrieved from: http://doi 10.1007/978-81-322-2006-0_2
13. (2016, November 30). An introduction to insecticides. . Retrieved from: http://ipmworld.umn.edu/ware-intro-insecticides
14. Jaramillo, M.C. , Arango, G. , Gonzalez, M.C. , Robledo, S.M. . (1999, November 30). Cytotoxicity and antileishmanial activity of Annona muricata pericarp. Filoterapia.. . Retrieved from: https://www.researchgate.net/publication/12587820_Cytotoxicity_ and_antileishmanial_activity_of_Annona_muricata_pericarp
15. Kamanula, J. , Sileshi, G.W. , Belmain, S.R. , Sola, P. , Mvumi, B.M. , Nyirenda, G.K.C. , Nyirenda, S.P.N. , Stevenson, P.C. . "Farmers’ insect pest management practices and pesticidal plant use in the protection of stored maize and beans in Southern Africa." International Journal of Pest Management 57(1): 41-49, 2011. Retrieved from: http://projects.nri.org/adappt/docs/kamanula_eta_2010.pdf
16. Kesetyaningsih, T. W. . "Efficacy of Annona squamosa leaf extract as an insecticide against Cockroach (Periplaneta Americana)"  International Conference: Research and Application of Traditional Complementary and Alternatice Medicine in Health care (TCAM), 2012. . Retrieved from: http://https://publikasiilmiah.ums.ac.id/handle/11617/2430
17. Kim, T. , Rust,  M. K. . "Life History and Biology of the invasive Turkestan cockroach (Dictyoptera: Blattidae)" Journal of Economic Entomology 106(6): 2428-2432, 2013. Retrieved from: http://www.bioone.org/doi/abs/10.1603/EC13052
18. Kovacic, P. , Somanathan, R.  . "Propoxur: a novel mechanism for insecticidal action and toxicity. " Reviews of environmental contamination and toxicology 218, 141-150, 2012. Retrieved from: http://doi:10.1007/978-1- 4614-3137-4_4
19. Llanos, C. A. H. , Arango, D. L. , Giraldo, M. C. . "Insecticidal activity of seed extracts of Annona muricata (Annonaceae) on Sitophilus zeamais (Coleoptera: Curculionidae)." Colombian Journal of Entomology 34(1): 2008. Retrieved from: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120- 04882008000100010
20. Moghadamtousi, S. Z. , Fadaeinasab, M. , Nikzad, S. , Mohan, G. , Ali, H. M. , Kadir, H. A. . "Annona muricata (Annonaceae): A review of its traditional uses, isolated Acetogenins and Biological activities." International Journal of Molecular Sciences 16, 15625-1565, 2015. Retrieved from: http://doi:10.3390/ijms160715625
21. Mulia, K. , Krisanti, E. , Maulana, T. , Dianursanti, D. . "Selective polarity-guided extraction and purification of Acetogenins in Annona muricata L. leaves." International Journal of Technology 6(7): 1221-1227, 2015. Retrieved from: http://https://www.researchgate.net/publication/288857178_Selective_Po larity-guided_Extraction_and_Purification_of_Acetogenins_in_ Annona_muricata_L_Leaves
22. Ogg, B. , Ogg, C. , Ferraro, D. . (2005, November 30). Cockroach Control Manual. (2nd ed.). . Retrieved from: http://lancaster.unl.edu/pest/roach/cockroach%20manual.pdf
23. Pirie, M. D. , Chatrou, L. W. , Erkens, R. H. J. , Maas, J. W. , Van derNiet, T. , Mols, J. B. , Richardson, J. E. . (2004, November 30). Phylogeny reconstruction and molecular dating in four Neutropical genera of Annonaceae: The effect of taxon sampling in age estimations.. . Retrieved from: https://www.researchgate.net/publication/224969142_Phylogeny_reconstruction_and_molecular_dating_in_four_Neotropi cal_genera_of_Annonaceae_The_effect_of_taxon_sampling_in_age_estimations
24. Potter, M. F. , Beavers, G. M. . (2004, November 30). Public health pest management: A training guide.. . Retrieved from: pest.ca.uky.edu/PSEP/Manuals/ent63.pdf
25. Quisumbing, E. . Medicinal plants of the Philippines Caloocan City: Kath, 1978.
26. Rust, M. K., , Reierson, D. A. . (2006, November 30). Cockroaches.. . Retrieved from: http://ipm.ucanr.edu/PMG/PESTNOTES/pn7467.html
27. Shimadzu Corporation, . (). Tips for practical HPLC analysis: Separation know-how.. . Retrieved from: https://www.shimadzu.eu/sites/default/files/Tips_for_practical_HPL C_analysis-Separation_Know-how.pdf
28. Tacio, H. D. . (2008, November 30). Guyabano: The fruit that heals.. . Retrieved from: https://wakeuphils.wordpress.com/2009/08/14/guyabano-the-fruit-that-heals/
29. Takada, M. , Kuwabara, K. , Nakato, H. , Tanaka, A. , Iwamura, H. , Miyoshi, H. . "Definition of crucial structural factors of acetogenins, potent inhibitors of mitochondrial complex I. " Biochimica et Biophysica Acta (BBA)-Bioenergetics 1460(1): 302-310, 2000. Retrieved from: http://dx.doi.org/10.1016/S0005-2728(00)00156-0
30. Taylor, L. . (2004, November 30). Technical data report for Graviola (Annona muricata). . Retrieved from: http://www.rain- tree.com/reports/graviola-techreport.pdf
31. Tellez, A. V. , Gonzalez, E. , Vazquez, E. V. , Yahia, E. M. . "Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity." Arabian Journal of Chemistry , 2016. Retrieved from: http://www.ksu.edu.sa http://dx.doi.org/10.1016/j.arabjc.2016.01.004
32. Texas A&M University, . (2015, November 30). Landscape IPM. . Retrieved from: http://landscapeipm.tamu.edu/types-of-pest- control/chemical-control/synthetic/
33. Tolosa, D. , Colom, O. A. , Bardon, A. , Neske, A. . "Insecticidal Effects of Acetogenins from Rollinia occidentalis Seed Extract." Natural Product Communications 7(12): 1645-1646, 2012. Retrieved from: http://www.researchgate.net/publication/235645806
34. Westra, L. Y. T. , Maas, P. J. M. . "Tetrameranthus (Annonaceae) revisited including new species." Photokeys 12, 1-21, 2012. Retrieved from: http://https://doi.org/10.3897/phytokeys.12.2771
35. World Health Organiz, . (). Propoxur: 2-isopropoxyphenyl methylcarbamate.. . Retrieved from: http://www.who.int/whopes/quality/en/propoxur_eval_spec_WHO_ October_2005.pdf
36. World Health Organiz, . (2007, November 30). Pesticides. . Retrieved from: http://www.who.int/ceh/capacity/Pesticides.pdf
37. Wurangian, F.L. . "Determination of Annonacyn Compound by highperformance liquid Chromatogrpahy on the extract of Annona muricata Linn seed for pesticide Formula." Indonesian Journal of Chemistry 5(3): 215-218, 2005. Retrieved from: http://pdm- mipa.ugm.ac.id/ojs/index.php/ijc/article/viewArticle/217
38. Yang, H. , Zhang, N. , Zeng, Q. , Yu, Q. , Ke, S. , Li, X. . " HPLC method for the simultaneous determination of ten Annonaceous Acetogenins after superficial fluid CO2 extraction." International Journal of Biomedical Science 6(3): 202-207, 2010. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC3615266/

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors U8 In7 2017 Fulltext Print Format

©2022 HERDIN PLUS. All rights reserved. | Contact Us | Keep up to date