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Potential of cassava (Manihot esculenta) starch as serum separator gel for glucose testing.

Author

Rhandonn L. Aga,
Jessa Christine F. Almerino,
Sam Nikkalai P. Baguio,
Merrel Rouke S. Cericos,
Carmel Therese P. Del Rosario,
Leigh Divina P. Ouano,
Allanys Joy Q. Pino,
Catrizze C. Saab,
Beatrice Andrea  C. Sievert,
Chelsea Lauren T. Soco,
Filconey Rose B. Camado

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Medical Technology
Publication Date
October 2016

Abstract

        The study conducted intended to determine the potential of cassava (Manihot esculenta) starch-derived gel as an alternative to the commercially synthesized thixotropic gel used for glucose testing. A repeated measures design was utilized for the implementation of the study. Blood samples were extracted from selected thirty-seven (37) respondents ranging from 18 to 25 years old, who were currently enrolled in Cebu Doctors' University. The first procedure done was the preparation of the cassava tuber extract using a high speed blender and the isolation of the starch component through filtration; 23 g of the starch isolated was weighed and added with 100 ml Normal Saline Solution (NSS) to make 0.23 g/ml of solution which underwent gelatinization through heating. Two experimental groups of different volumes were prepared in the study--0.5 ml and 1.0 ml. Twelve (12) ml of blood was extracted using the venipuncture syringe method from each respondent and equally dispensed in three tubes--tube with 0.5 ml cassava starch gel, with 1.0 ml cassava starch gel, and the commercial serum separator tube with the thixotropic gel which served as the control group of the study. Only the tubes which showed successful separation of the blood components were utilized for glucose testing.


        Based on the results, both volumes of cassava starch-derived gel were both capable of separating the cellular components from the serum with both having a percentage of 86.49%.Tubes with blood samples that had failed to separate may have been caused by incomplete clotting due to improper timing of centrifugation which is no longer than 2 hours after collection. Becton, Dickinson and Company, n.d.).The mean glucose levels of the control group and the experimental groups were computed and the result of the One Way Repeated measures ANOVA showed a significant difference between the experimental groups and the control group. On the average, the mean glucose level of blood samples taken from the commercial serum separator tube with thixotropic gel was significantly higher compared with those from tubes with 0.5 ml cassava starch gel and 1.0 ml cassava starch gel. On the other hand, there was no significant difference in the blood glucose levels of both volumes in the experimental groups.


         In conclusion, both volumes of the cassava starch gel were effective in separating the blood components but yielded lower mean blood glucose levels compared to that of the commercial serum separator tube due to resistant starch which has the property of decreasing blood glucose levels. Despite its ability to separate the blood components, the cassava starch gel did not have any potential as an alternative serum separator gel for glucose testing. Thus, use of cassava starch gel in measuring other blood analytes such as total cholesterol is recommended. 

References

1. Aldana, D.G. , Tanagon, S.A. ,  Manantan, R.G. , Pudlao,  N.A. , Valenciano,  J.D. , Fabro , R.L. , Espiritu, A.C. . "Potato starch extract asan alternative serum separator gel and its effects on glucosetesting." Northern Luzon Research Journal 2, 93-103, 2008. Retrieved from: http://pejard.slu.edu.ph/images/pdf/vol2/potatostarch-extract-as-an-alternative-serum-separator-gel-and-itseffects-on-glucose-testing.pdf
2. Anbuselvi, T. , Balamurugan, S. . "Phytochemical and antinutrientconstituents of cassava and sweet potato." World Journal of  Pharmacy and Pharmaceutical Sciences 3(3): 1440-1449, 2014. Retrieved from: http://fromwww.wjpps.com/download/article/1396443500.pdf
3. Ashworth, C.T. , Adams, G.  . "Blood specific gravitystudies" Translational Research: The Journal ofLaboratory and Clinical Medicine 26(12): 1934-1939, September 1941. Retrieved from: http://www.translationalres.com/article/S0022-2143(41)90044-6/pdf
4. Becton, , Dickinson & Company, . (). Product FAQs. . Retrieved from: http://www.bd.com/vacutainer/faqs/
5. Benesi, R.M.I. , Labuschagne, M.T. , Dixon, A.G.O. . "Stability of native starch quality parameters, starch extraction and root dry matter of cassava genotypes in different environments." Journal of the Science of Food and Agriculture 84(11): 1381-1388, 2004. Retrieved from: http://DOI: 10.1002/jsfa.1734
6. Booth, K.A. , Mundt, L. . Phlebotomy: A competency-basedapproach. New York: McGraw-Hill, 2013.
7. Bowen, R.A.R. , Remaley, A.T. . "Interferences from bloodcollection tube components on clinical chemistry assays." BiochemiaMedica 24(1): 31-44, 2014. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936985/pdf/biochem-24-1-31-7.pdf
8. Boyle, J.E. , Glasscock, G.C. . U.S. Patent No. 3,878,196 Washington, DC: U.S. Patent and Trademark Office, 1975. . Retrieved from: http://https://docs.google.com/viewer?url=patentimages.storage.googleapis.com/pdfs/US3878196.pdf
9. Carey, C.L. . "A method of preparation and some properties of astarch gel." Bulletin of the Torrey Botanical Club 47(10): 455-463, 1920. Retrieved from: http://https://www.jstor.org/stable/2480198?seq=1#page_scan_tab_contents
10. Castro, L. , De Guzman, A. , Padua, R. , Supan, E. , Tan, C. , Yauaf, P. , Nieto, X. . "The serum separating capacity of ipomoeabatatas starch in the routine glucose determination. " International Journal of Scientific and Research Publications 5(6): 1-3, 2015. Retrieved from: http://www.ijsrp.org/research-paper-0615/ijsrp-p4256.pdf
11. Conte, R.L. . (). Nutrition per Hectare for staple crops: Protein, fat,carbohydrates, calories.. . Retrieved from: http://www.gardeningplaces.com/articles/nutrition-per-hectare1.htm
12. Ebuehi, O.A.T. , Babalola, O. , Ahmed, Z. . "Phytochemical, nutritiveand anti-nutritive composition of cassava (Manihot esculenta L)tubers and leaves" Nigerian Food Journal 23(1): 40-46, 2005. Retrieved from: http://www.ajol.info/index.php/nifoj/article/view/33597
13. Fakir, M.S.A , Jannat, M. , Mostafa, M.G, , Saal, H, . "Starch andflour extraction and nutrient composition of tuber in seven cassavaaccessions." Journal of the Bangladesh Agricultural University 10(2): 217-222, 2012. Retrieved from: http://www.banglajol.info/index.php/JBAU/article/view/14698/10441
14. Fatás, M. , Franquelo, P.  , Franquelo, R. . "Anomalous flotation of separator gel: density or viscosity." Clinical Chemistry 54(4): 771-772, 2008. Retrieved from: http://clinchem.aaccjnls.org/content/54/4/771.long
15. Fiehler, W.R. . U.S. Patent No. 4,946,601 Washington, DC: U.S.Patent and Trademark Office, 1990. . Retrieved from: http://www.google.com.ph/patents/US4946601
16. Galvez, J. , Pua, M. , Bercasio, J. , Chen, S. , Chua, R. , Cristobal, X. , Nieto, X. . "The serum separating capacity of ipomoeabatatas starch in creatinine and total cholesterol determination." International Journal of Scientific and Research Publications 5(12): 308-310, 2015. Retrieved from: http://www.ijsrp.org/research-paper-1215/ijsrp-p4847.pdf
17. Gunnars, K. . (). Resistant starch 101 - Everything you need to know.. Authority Nutrition. . Retrieved from: https://authoritynutrition.com/resistant-starch-101/
18. International Starch, . (). Cassava. . Retrieved from: http://www.starch.dk/isi/starch/cassava.asp
19. Johnson-Davis, K.L. . Enzymes. Clinical chemistry: Techniques, principles, andcorrelations (7th ed., Chapter 13) Philadelphia: WoltersKluwer/Lippincott Williams & Wilkins., 2013. p. 283-285.
20. Kresser, C. . (2013, November 30). How resistant starch will help to make you healthier and thinner.. . Retrieved from: https://chriskresser.com/how-resistant-starch-will-help-to-makeyou-healthier-and-thinner/
21. McCall, R,E. , Tankersley, C.M. . Phlebotomy essentials (4thed.) Baltimore: Lippincott Williams & Wilkins, 2008.
22. Mullins, C.A. . Specimen collection. Hematology: Clinical principles andapplications (4th ed.) Singapore: Elsevier, 2012. p. 20.
23. National Institute f, . (2014, November 30). Silica...It. . Retrieved from: https://www.cdc.gov/niosh/docs/97-118/
24. Ott, M. , Hester, E.E . (1963, November 30). Gel formation as related toconcentration of amylose and degree of starch swelling. . Retrieved from: http://www.aaccnet.org/publications/cc/backissues/1965/Documents/chem42_476.pdf
25. Pereira, B.L.B. , Leonel, M. . "Resistant starch in cassava products." Food Science and Technology (Campinas) 34(2): 298-302, 2014. Retrieved from: http://www.scielo.br/pdf/cta/v34n2/aop_cta_6270.pdf
26. Robertson, . "Dietary-resistant starch and glucose metabolism" Current Opinion in Clinical Nutrition and Metabolic Care 15(4): 362-367, 2012. Retrieved from: http://https://www.ncbi.nlm.nih.gov/pubmed/22510681
27. Tako, M. , Tamaki, Y. , Teruya, T. , Yakeda, Y. . "The principles ofstarch gelatinization and retrogradation." Food and Nutrition Sciences 5(03): 280-291, 2014. Retrieved from: http://doi:10.4236/fns.2014.53035
28. Tewe, O.O. . (2003, November 30). Cassava for livestock feed in sub-Saharan Africa.Food and Agriculture Organization of the United Nations (FAO). . Retrieved from: ftp://ftp.fao.org/docrep/fao/007/j1255e/j1255e00.pdf
29. Thixotropic. American Heritage® Dictionary of the EnglishLanguage (5th ed.) 2016. . Retrieved from: http://www.thefreedictionary.com/thixotropic
30. Trudnowski, R.J. , Rico, R.C. . "Specific gravity of blood and plasmaat 4 and 37ºC " Clinical Chemistry 20(5): 615-616, 1974. Retrieved from: http://www.researchgate.net/publication/18341615_Specific_Gravity_of_Blood_and_Plasma_at_4_and_37_C
31. Yamada, Y. , Hosoya, S. , Nishimura, S. , Tanaka, T. , Kajimoto, Y. , Nishimura, A. , Kajimoto, O. . "Effect of bread containingresistant starch on postprandial blood glucose levels inhumans." Bioscience, Biotechnology, and Biochemistry 69(3): 559-566, 2005. Retrieved from: http://https://www.jstage.jst.go.jp/article/bbb/69/3/69_3_559/_pdf

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U4 P848ag 2016 Fulltext Print Format

 
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