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Thrombocytosis effect of Carica papaya seed extraction on the platelet count of aspirin-induced albino rabbit (Oryctolagus cuniculus).


Eunice G. Abucay,
Joana Marie U. Ancheta,
Carl Robyn C. Deiparine,
Evangel Claudette L. Domapias,
Tricia Louise S. Ongchua,
Shainna Noelle V. Tan,
Reinna Jean R. Toong,
Jolina Ghea O. Uy,
Filconey Rose B. Camado

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Medical Technology
Publication Date
October 2016


This study aimed to determine the ability of Carica papaya seed extract to increase the platelet count of aspirin-induced thrombocytopenic albino rabbits. The experimental method used was a randomized controlled trial, single blind. Fifteen (15) male albino rabbits were divided into three (3) groups, composed of five (5) rabbits each. Aspirin was administered to the subjects for a period of one (1) week in order to simulate a thrombocytopenic state. Carica papaya seed extract was obtained by subjecting dried papaya seeds to ethanol extraction with the use of the Soxhlet apparatus and Rotavap. The three (3) groups received different dosages of the Carica papaya extract for fourteen (14) days: experimental group 1 received 0.5 mL/kg of seedextract, experimental group 2 received 1 mL/kg of the extract, and the negative control group did not receive any extract, only distilled water. Indirect platelet counting was done during the 7th and 14th days.

The findings of the study demonstrated a significant difference across time in all three groups. Using Repeated Measures ANOVA, it was found that for the 0.5 mL/kg Carica papaya group, aspirin-induced platelet count was significantly lower compared with day 7 post-treatment by 207,345 plt/μL, but day 7 and day 14 post-treatment platelet counts were the same. For the 1 mL/kg Carica papaya group, aspirin-induced and day 7 post-treatment platelet counts were the same. However, day 7 post-treatment platelet count was significantly lower compared with day 14 post-treatment by 103,862.2 plt/μL. The distilled water group also showed a significantly lower aspirin-induced platelet count compared with day 7 post-treatment by 128,221.2 plt/μL. However, day 7 and day 14 post-treatment platelet counts were the same. One-way ANOVA of day 7 post-treatment platelet counts showed that there was no significant difference between the three groups, indicated by a p value of 0.535. Day 14 post-treatment platelet counts also did not show a significant difference across treatment groups, shown by a p value of 0.341.

In conclusion, since the control and experimental groups did not exhibit a significant difference after seed extract administration, it can be inferred that Carica papaya seed extract was not an effective anti-thrombocytopenic agent during 7th and 14th day post-treatment. The researchers recommend that the time intervals between extractions and platelet counts be shortened to under a week, in order to properly determine whether the increase in platelet count is due to the Carica papaya seed extract or merely the body's natural mechanism of replenishing platelets.


1. (2015, November 30). 10 low platelet count causes (Disease-induced). . Retrieved from: http://www.thrombocyte.com/cause-low-platelet-count/
2. American Society of , . (2015, November 30). Aspirin. . Retrieved from: https://medlineplus.gov/druginfo/meds/a682878.html
3. Arollado, E. C. , Peña, I.G. , Dahilig, V.R.A. . "Platelet augmentation activity of selected Philippine plants" International Journal of Pharmaceutical and Phytopharmacological Research 3(2): 2013. Retrieved from: http://eijppr.com/vol3-issue2/eIJPPR-2013-3-2-121-123.pdf
4. Aziz, J. , Kassim, N.L.A. , Kasim, N.H.A. , Haque, N. , Rahman, M.T. . "Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells" BMC Complementary and Alternative Medicine 15(1): 2015. Retrieved from: http://doi: 10.1186/s12906-015-0749-6
5. Burdick, E. M. . "Carpaine: An alkaloid of Carica papaya: Its chemistry and pharmacology" Economic Botany 25(4): 363–365, 1971. Retrieved from: http://www.jstor.org/stable/4253284
6. Castañeda, C. R. , Etemadi, J.O. , Evangelista, C.I.C. , Prieto, J.A. , Sanchez, M.A. , Suarez, K.R.I. . The effect of Euphorbia hirta (mangagaw) leaf and stalk decoction on Oryctolagus cuniculus50(rabbits) with aspirin-induced thrombocytopenia . Mandaue City. 2007. Cebu Doctors,
7. Centers for Disease , . (2015, November 30). Dengue. . Retrieved from: http://www.cdc.gov/dengue/
9. Delphin, D. V , Haripriya, R. , Subi, S. , Jothi, D. , Vasan, P. T. . "Phytochemical screening of various ethanolic seed extracts" World Journal of Pharmacy and Pharmaceutical Sciences 3(7): 1041–1048, 2014. Retrieved from: http://webcache.googlesuercontent.com/search?q=cache:8lJDWirduyMJ:www.wjpps.com/download/article/1404207796.pdf+&cd=1&hl=en&ct=clnk&gl=ph
10. Dharmarathna, S. L. C. A. , Wickramasinghe, S. , Wauge, R. N. , Rajapakse, R. P. V. J. , Kularantne, S. A. M. . "Does Carica papaya leaf-extract increase the platelet count?: An experimental study in a murine model" Asian Pacific Journal of Tropical Biomedicine 3(9): 720-724, 2013. Retrieved from: http://doi: 10.1016/S2221-1691(13)60145-8
11. Edillo, F.E. , Halas, Y.A. , Largo, F.M. , Erasmo, J.N.V. , Amoin, N.B. , Alera, M.T.P. , Shepard, D.S. . "Economic cost and burden of dengue in the Philippines" The American Journal of Tropical Medicine and Hygiene 92(2): 360-366, 2015. Retrieved from: http://doi: 10.4269/ajtmh.14-0139
12. Fritsma, G. . An overview of clinical laboratory hematology. Hematology: Clinical principles and applications (3rd ed.,) Singapore: Elsevier, pp. 2-6.
13. Fritsma, G. . Platelet production, structure, and function. Hematology: Clinical principles and applications (3rd ed., ) Singapore: Elsevier, pp. 152-16.
14. Harcourt-Brown, F. . (2001, November 30). Textbook of rabbit medicine. . Retrieved from: http://krolikdoma.com/forum/attachments/textbook-of-rabbit-medicine-pdf.1342/
15. Hayatie, L. , Biworo, A. , Suhartono, E. . "Aqueous extracts of seed and peel of Carica Papaya against Aedes Aegypti" Journal of Medical and Bioengineering 4(5): 417–421, 2015. Retrieved from: http://doi:10.12720/jomb.4.5.417-421
16. (). Hematology in rabbits and guinea pigs. . Retrieved from: http://www.laboklin.de/pdf/en/aktuell/lab_akt_0910_en.pdf
17. (2015, November 30). Hematology: Platelet count. . Retrieved from: https://www.vetstream.com/clinical-reference/lapis/labtest/hematolog y-platelet-count#resultdata
18. Ikpeme, E. V. , Kooffreh, M.E. , Udensi, O. . "Phytochemistry and heamatological potential of ethanol seed leaf and pulp extracts of Carica papaya (Linn.)" Pakistan Journal of Biological Sciences 14(6): 408-411, . Retrieved from: http://doi: 10.3923/pjbs.2011.408.411
19. Imaga, N. O. A. , Gbenle, G.O. , Okochi, V.I. , Akanbi, S.O. , Edeoghon, S.O. , Oigbochie, V. , Bamiro, S.B. . "Antisickling property of Carica papaya leaf extract." African Journal of Biochemistry Research 3(4): 102-106, 2009. Retrieved from: http://www.academicjournals.org/journal/AJBR/article-full-text-pdf/499890910533
20. Lashin, I. I. , Elhaw, M.H. . "Evaluation of secondary metabolites in callus and tissues of Physalis peruviana" International Journal of Modern Botany 6(1): 10-17, 2016. Retrieved from: http://doi:10.5923/j.ijmb.20160601.03
21. Lee, J. , Kim, J.K. , Kim, J.H. , Dunuu, T. , Park, S.H. , Hyon, M.S. . "Recovery time of platelet function after aspirin withdrawal" Current Therapeutic Research 76, 26-31, 2014. Retrieved from: http://doi: 10.1016/j.curtheres.2014.02.002
22. Lohiya, N. K. , Goyal, R.B. , Jayaprakash, D. , Ansari, A.S. , Sharma, S. . "Antifertility effects of aqueous extract of Carica papaya seeds in male rats" Planta medica 60(5): 400-404, 1994. Retrieved from: http://doi: 10.1055/s-2006-959518
23. Marangos, M.N. , Onyeji, C.O. , Nicolau, D.P. , Nighttingale, C.H. . "Disposition kinetics of aspirin in female New Zealand white53rabbits" Laboratory Animal Science 45(1): 67-69, 1995. Retrieved from: http://https://www.researchgate.net/publication/15445810_Disp osition_kinetics_of_aspirin_in_female_New_Zealand_white_rabbits
24. Marfo, E.K. , Oke, O.L. , Afolabi, O.A. . "Chemical composition of papaya (Carica papaya) seeds" Food Chemistry 22, 259-266, 1986. Retrieved from: http://doi: 10.1016/0308-8146(86)90084-1
25. Martel, J. . (2015, November 30). Low platelet count (Thrombocytopenia). . Retrieved from: http://www.healthline.com/desktop-article/thrombocytopenia?variation=nolb#Overview1
26. Nayak, M.K. , Dash, A. , Singh, N. , Dash, D. . "Aspirin delimits platelet life span by proteasomal inhibition" PLoS ONE 9(8): 2014. Retrieved from: http://doi:10.1371/journal.pone.0105049
27. Nwangwa, E.K. , Ekhoye, E.I , Ugoji, A.E. . "A study of haemato-stimulatory effects of graded dose of aqueous Carica papaya seed extract in Wistar rats" Research Journal of Pharmaceutical, Biological and Chemical Sciences 4(1): 129-136, 2013. Retrieved from: http://www.rjpbcs.com/pdf/2013_4(1)/[15].pdf
28. Okeniyi, J.A. , Ogunlesi, T.A. , Oyelami, O.A. , Adeyemi, L.A. . "Effectiveness of dried Carica papaya seeds against human intestinal parasitosis: A pilot study." Journal of Medicinal Food 10(1): 194-196, 2007. Retrieved from: http://doi: 10.1089/jmf.2005.065
29. Olagunju, J.A. , Adeneye, A.A. , Fagbohunka, B.S. , Bisuga, N.A. , Ketiku, A.O. , Benebo, A.S. , Adeleke, A.G. . "Nephroprotective54activities of the aqueous seed extract of Carica papaya Linn. in carbon tetrachloride induced renal injured Wistar rats: A dose- and time-dependent study" Biology and Medicine 1(1): 2009. Retrieved from: http://biolmedonline.com/Articles/vol1_1_11-19.pdf
30. Sarala, N. , Paknikar, S.S. . "Papaya extract to treat dengue: A novel therapeutic option?" Annals of Medical and Health Sciences Research 4(3): 320-324, 2014. Retrieved from: http://doi: 10.4103/2141-9248.133452
31. Sellahewa, K. H. . "Pathogenesis of dengue haemorrhagic fever and its impact on case management" ISRN Infectious Diseases, 2013. , 2013. Retrieved from: http://doi: http://dx.doi.org/10.5402/2013/571646
32. Sheikh, N. , Younas, N. , Akhtar, T. . " Effect of Carica papaya leaf formulation on hematology and serology of normal rat" Biologia (Pakistan) 60(1): 139-142, 2014. Retrieved from: http://biosoc.pk/wp-content/uploads/2014/10/20.-19-14-Papaya. pdf
33. (2015, November 30). The surprising health benefits of papaya seeds. . Retrieved from: http://superfoodprofiles.com/health-benefits-papaya-seeds
35. Yunita, F. , Hanani, E. , Kristianto, J. . "The effect of Carica papaya L. leaves extract capsules on platelets count and hematocrit level in dengue fever patient" International Journal of Medicinal and Aromatic Plants 2(4): 573-578, 2012. Retrieved from: http://www.openaccessscience.com/pdf-files/vol2_4_dec2012//IJMAP _2_4_4_Carica_papaya.pdf
36. Zunjar, V. , Dash, R.P. , Jivrajani, M. , Trivedi, B. , Nivsarkar, M. . "Antithrombocytopenic activity of carpaine and alkaloidal extract of Carica papayalinn. leaves in busulfan induced thrombocytopenic Wistar rats" Journal of Ethnopharmacology,181 , 2016. Retrieved from: http://doi: 10.1016/j.jep.2016.01.035

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