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Anti-lipidemic activity of NSIC RC 336 rice bran extract on total cholesterol and triglyceride levels in albino rats.


Derrick Acasio,
Ma. Avegail Bolarde,
Katrina Caumeran,
Dominic Anthon Lopez,
Daniel Malingin,
Shine Clarence Signe,
Joyce Torrefranca,
Filconey Rose B. Camado

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Medical Technology
Publication Date
October 2015


     The research was about the lowering effect of the NSIC RC 336 Rice Bran extract on the total cholesterol and triglyceride levels of the albino rats. This study is a quantitative experimental research design wherein randomized
controlled trial was used. The extraction of the rice bran was conducted at the Cebu Doctor's University Research Laboratory. The rice bran extraction was completed under the supervision of an assigned laboratory technician. The
experimentation phases of the study were conducted at the Cebu Doctor's Animal Laboratory and the researchers were guided by a licensed veterinarian and licensed animal caretaker under the supervision of Institutional Animal Care and Use Committee (IACUC). Determination of all the total cholesterol and triglyceride levels were done at the Cebu Doctor's University Diagnostics Center with the use of spectrophotometer.

     The albino rats were acclimatized under laboratory conditions for fifteen (15) days (light period 8:00 A.M. to 8:00 P.M., 20 ± 3 ºC). Blood was collected three (3) times for the quantitation of the specific analytes used in the study. First, blood was collected for the mean baseline level after the subjects underwent fasting. Second, collection was done after inducing a high fat diet. Third, it was collected after the treatment phase was finished. The tail vein was used as the site of blood collection for the first and second extraction while cardiac puncture was
done for the third blood extraction.

    The findings of the study established that the total cholesterol levels were significantly lowered after treatment with the rice bran extract by 43% and a 54% decrease using the treatment tocopherol. When compared to the no treatment group, it showed that the results for rice bran extract group and tocopherol group had lower total cholesterol by 23.0 mg/dl and 31.64 mg/dl respectively. On the contrary, rice bran extract lowered the triglyceride levels only by 9% compared to the tocopherol which decreased the triglyceride level by 43%. Statistically, the
rice bran extract group had lower triglyceride levels than the no treatment group although it was not significant. There was no enough evidence that the rice bran extract can effectively decrease the triglyceride levels. Therefore, the 100 mg/kg rice bran extract significantly lowered the total cholesterol compared to the triglyceride levels. The recommendation of this study is directed towards the use of natural ways of lowering cholesterol and triglycerides and to modify the method and concentration of the rice bran to show its capacity to lower the
triglyceride levels.


1. Asian Bariatrics, . (). The challenges of obesity: Morbid obesity presents clear and big risks to our health, Filipino expert says. . . Retrieved from: http://asianbariatrics.rxpinoy.com/new_challenge.html#top
2. Bishop, M. L. , Fody, E. P. , Schoeff, L. E. (Eds.) . Clinical Chemistry: principles,techniques, and correlations. Philadelphia: Lippincott Williams & Wilkins, 2013.
3. Breuer, H. W. M. . "Hypertriglyceridemia: A review of clinical relevance andtreatment options: Focus on cerivastatin." Current Medical Research and Opinion 17(1): 60-73, 2001. Retrieved from: http://www.medscape.com/viewarticle/407814_6
4. Burnett, J. R. . "Lipids, lipoproteins, atherosclerosis and cardiovascular disease." The Clinical Biochemist Reviews 25(1): 2, 2004. Retrieved from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1853363/
5. Cicero, A. F. G. , Derosa, G. . "Rice bran and its main components: Potential rolein the management of coronary risk factors." Current Topics in Nutraceutical Research 3(1): 29-46, 2005. Retrieved from: http://ctnr.newcenturyhealthpublishers.com/about/pdf/issue_3_1_29-46.pdf
6. Department of Agricu, . (). National Seed Industry Council seed catalogue: Crop varieties 2012. . Retrieved from: http://nseedcouncil.bpinsicpvpo.com.ph/downloadables/catalogue2013.pdf
7. Department of Agricu, . (). Improved rice. . Retrieved from: http://philrice.gov.ph/about-us/rice-science/our-work/
8. Gajda, A. M. . (2008, November 30). Obesity: High fat diets for diet-induced obesity models. . . Retrieved from: file:///D:/My%20Documents/Downloads/Obesity%20(4).pdf
9. Ghatak, S. B. , Panchal, S. J. . " Anti-hyperlipidemic activity of oryzanol, isolatedfrom crude rice bran oil, on Triton WR-1339-induced acute hyperlipidemia in rats." Brazilian Journal of Pharmacognosy , 2012. Retrieved from: http://doi:10.1590/S0102-695X2012005000023
10. Hasselwander, O. , Kramer, K. , Hoppe, P. P. , Oberfrank, U. , Baldenius, K. , Schroder, H. . " Effects of feeding various tocotrienol sources on plasma lipids and aorticatherosclerotic lesions in cholesterol-fed rabbits." Food Research International 35(2): 245-251, 2002. Retrieved from: http://doi:10.1016/S0963-9969(01)00192-2
11. Incardona, J. P. , Eaton, S. . Cholesterol in signal transduction. CurrentOpinion in Cell Biology 12(2): 193-203, 2000. Retrieved from: http://doi:10.1016/S0955-0674(99)00076-9
12. Lai, M. H. , Chen, Y. T. , Chen, Y. Y. , Chang, J. H. , Cheng , H. H. . " Effects of rice bran oil on the blood lipids profiles and insulin resistance in type 2 diabetes patients." Journal of Clinical Biochemistry and Nutrition 51(1): 15, 2012. Retrieved from: http://doi:10.3164/jcbn.11-87
13. Marubayashi, S. , Dohi, K. , Ochi, K. , Kawasaki, T. . "Role of free radicals inischemic rat liver cell injury: prevention of damage by alpha-tocopheroladministration." Surgery 99(2): 184-192, 1986. Retrieved from: http://doi:10.1172/JCI113441
14. Minhajuddin, M. , Beg , Z. H. , Iqbal, J. . "Hypolipidemic and antioxidantproperties of tocotrienol rich fraction isolated from rice bran oil in44experimentally induced hyperlipidemic rats. " Food and ChemicalToxicology 43(5): 747-753, 2005. Retrieved from: http://doi:10.1016/j.fct.2005.01.015
15. Oliveira, R. , Oliveira, V. , Aracava, K. K. , da Costa Rodrigues, C. E. . "Effects of the extraction conditions on the yield and composition of rice branoil extracted with ethanol—A response surface approach." Food and Bioproducts Processing 90(1): 22-31, 2012. Retrieved from: http://https://getinfo.de/en/search/id/elsevier%3Adoi~10.1016%252Fj.fbp.2011.01.004/Effects-of-the-extraction-conditions-on-the-yield/
16. Patel, J. , Sheehan, V. , Gurk-Turner, C. . "Ezetimibe (Zetia): a new type oflipid-lowering agent. " Proceedings of Baylor University Medical Center 16(3): 354, 2003. Retrieved from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1200795/
17. Patel , M. , Naik, S. N. . "Gamma-oryzanol from rice bran oil: a review." Journal of Scientific and Industrial Research 63, 569-578, 2004. Retrieved from: http://nopr.niscair.res.in/bitstream/123456789/5457/1/JSIR%2063%287%29%20569-578.pdf
18. Qureshi, A. A. , Mo, H. , Packer, L. , Peterson, D. M. . " Isolation andidentification of novel tocotrienols from rice bran with hypocholesterolemic,antioxidant, and antitumor properties." Journal of Agricultural and Food Chemistry 48(8): 3130-3140, 2000. Retrieved from: http://doi: 10.1016/j.lfs.2005.12.001
19. Rossouw, J. E. , Rifkind, B. M. . "Does lowering serum cholesterol levels lowercoronary heart disease risk?." Endocrinology and Metabolism Clinics of North America 19(2): 279-297, 1990. Retrieved from: http://europepmc.org/abstract/MED/2192874
20. Scavariello, E. M. , Arellano, D. B. . "Gamma-oryzanol: An important componentin rice brain oil. " Archivos latinoamericanos de nutricion 48(1): 7-12, 1998. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/9754398
21. World Health Organiz, . (2014, November 30). Obesity and overweight (Fact Sheet No. 311).. . Retrieved from: http://www.who.int/mediacentre/factsheets/fs311/en/

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