i
Report an error   
Advanced Search


Raw soybean (Glycine max): An alternative to nutrient agar as enrichment medium for Proteus species and Streptococcus pneumoniae.

Author

Mary Joy Alburo,
Hope Donnah Mae Dopeño,
Queenie Ferrolino,
Noelyn Fontanoza,
Heeney Grace Inoferio,
Mary Rose Omolon,
Carlven UY,
Kenneth Greg C. Cortes

Related Institution

College of Allied Medical Sciences - Medical Technology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Medical Technology
Publication Date
April 2015

Abstract

     This study determined the effectiveness of Raw Soybean Agar as an alternative enrichment medium to Nutrient Agar for Proteus species and Streptococcus pneumoniae. The researchers utilized a randomized post-test only experimental control group design. A total of forty (40) petri dishes were used in the study, twenty plates were that of Raw Soybean Agar and another twenty (20) for Nutrient Agar.Bacterial suspensions were prepared from the pure culture of the test organisms and was compared with that of zero point five (0.5) McFarland Standard to standardize the amount of bacteria present in the suspension. Pour Plate Method was performed to quantitate colonies that grew on the medium using a one is to twenty (1:20) dilution. Colonies on the medium were counted using a Quebec colony counter. Gram staining was performed to identify the morphology of the bacteria. Biochemical testing for Proteus species and Bile Solubility Test for Streptococcus pneumoniae were performed to confirm these test organisms.
      Results revealed comparable growth between the Raw Soybean Agar and the Nutrient Agar. Results showed that there was no significant difference in the colony count. Gram staining results revealed Gram-positive diplococci for Streptococcus pneumoniae and Gram-negative coccobacilli for the Proteus species. Bile Solubility Test confirmed that the colonies found on the Raw Soybean Agar and Nutrient Agar were Streptococcus pneumoniae. IMVIC results plus urease test confirmed that the colonies found on both the Nutrient Agar and Raw Soybean Agar was Proteus species.
     Statistical treatment of the data revealed that the number of colonies that grew on Raw Soybean Agar showed comparable results with that of the Nutrient agar, making it an effective alternative to Nutrient Agar as enrichment medium for Proteus species and Streptococcus pneumoniae. The researchers would like to recommend a clearing agent for easier visualization of colonies in Raw Soybean Agar due to the presence of particles in the agar. The researchers also recommend a pH regulator that will not interfere with the colony growth to produce a neutral pH in the medium. For future researchers, since pigmentation was produced when Streptococcus pneumoniae was cultured on Raw Soybean Agar, they may study on what could have caused it as well.

References

1. Agravante, K. , Aguilar, J. , Campos, A. , Catantan, A. , deGracia, A. , Godornes, M. , Miguel, R. , Veloso, C. . The effectivity of various concentration of mango (mangiferaindica) seed kernel extract as an antibacterial for escherichia coli-induced water sample.. Mandaue City. 2013. Cebu Doctors’ University,
2. Arulanantham, R. , Pathmanathan, S. , Ravimannan, N. , Niranjan, K. . "Alternative culture media for bacterial growth using different formulation of protein sources." Journal of Natural Product & Plant Resources 2(6): 697-700, 2012. Retrieved from: http://scholarsresearchlibrary.com/JNPPR-vol2-iss6/JNPPR-2012-2-6-697-700.pdf
3. Bois, C. M. D. , Mintz, S. W. . (2002, November 30). Encyclopedia of food and culture.. Encyclopedia.com. . Retrieved from: http://www.encyclopedia.com/topic/Soy.aspx#1-1G2:3403400548-full
4. Brooks, G. , Carroll, K. , Butel, J. , Morse, S. , Mietzner, T. . Jawetz, Melnick&Adelberg Singapore: McGraw-Hill, 2010.
5. Don, S. M. . (2008, November 30). Optimal conditions for the growth of E. Coli.. . Retrieved from: http://www.scribd.com/doc/11337868/Optimal-Conditions-for-the-Growth-of-E-Coli#scribd
6. Forbes, B. A. , Sahm, D. F. , Weissfeld, A. . Bailey and Scott’s Diagnostic Microbiology.12thed. Singapore: Elsevier, 2007.
7. (2007, November 30). Growing bacteria in vitro. . Retrieved from: http://virtuallaboratory.colorado.edu/BioFun-Support/labs/EColi%20introduced/section_02.html
8. Hartsock, A. . (2012, November 30). Growth requirements of E. coli and auxotrophs.. . Retrieved from: http://study.com/academy/lesson/growth-requirements-of-e-coli-and-auxotrophs.html#lesson
9. Hawwa, R. . (2014, November 30). Nutrient agar plate information.. . Retrieved from: http://www.livestrong.com/article/395181-nutrient-agar-plate-information/
10. Isin, S. H. . Regulation of catalase gene expression in soybean. United States. 1992. Texas Tech University, . Retrieved from: http://https://repositories.tdl.org/ttu-ir/bitstream/handle/2346/16159/31295007098394.pdf?sequence=1&isAllowed=y
11. National Center for , . (2012, November 30). Streptococcus pneumoniae.. Centers for Disease Control and Prevention . . Retrieved from: http://www.cdc.gov/pneumococcal/clinicians/streptococcus-pneumoniae.html
12. Patterson, K. , Bhagwat, S. , Williams , J. , Howe, J. , Holden, J. . (2007, November 30). USDA database for the choline content of common foods release two.. United States Department of Agriculture, Agricultural Research Service. . Retrieved from: http://www.ars.usda.gov/SP2UserFiles/Place/80400525/Data/Choline/Choln02.pdf
13. Presser, K. A. , Ratkowsky, D. A. , Ross, T. . "Modelling the growth rate of Escherichia colias a function of pH and lactic acid concentration." Applied and Environmental Microbiology 63(6): 2355-2360, 1997. Retrieved from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168528/
14. Quisumbing, R. T. . Laboratory manual in Bacteriology for Medical Technology students.. Mandaue City. 2000. Cebu Doctors’ University,
15. Restrepo, A. V. , Salazar, B. E. , Agudelo, M. , Rodriguez, C. A. , Suluaga, A. F. , Omar, V. . "Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae." BMC Microbiology 5(34): 2005. Retrieved from: http://www.biomedcentral.com/1471-2180/5/34
16. Uzeh, R. , Akinola, S. , Olatope, S. . "Production of peptone from soya beans (Glycine max L merr) and African locust beans (Parkiabiglobosa)." African Journal of Biotechnology 5(18): 1684-1686, 2006. Retrieved from: http://www.ajol.info/index.php/ajb/article/view/55823
17. Wang, Y. , Pan, X. . Proteus.. Encyclopedia of Food Safety Singapore: Academic Press, Elsevier, 2014.

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U4 R199 2015 Fulltext Print Format

 
Loading…
©2022 HERDIN PLUS. All rights reserved. | Contact Us | Keep up to date