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The antifungal effect of the sea urchin's (Tripneustes gratilla) coelomic fluid on Candida albicans.


Alec Josue  S. Bancale,
Edbert Glenn  L. Dy,
Ramon Miguel  M. Lastimosa,
Geneveve  A. Mendoza,
Kent Darwin  S. Ng Pineda,
Kattleya Anne  E. Ortizano,
David Duvin  C. Quijano,
Corazon V. Canapi

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
October 2015


              This study aimed to identify the antifungal effect of sea urchin's body fluid (Tripneustes gratilla) in Candida albicans. The study was conducted to find an alternative cure against Candida albicans.

                The present investigation elucidates the bioactive potential of aqueous extract of sea urchin Tripneustes gratilla. The Sabouraud agar plates were seeded with the inoculum of the used sterile cotton swab. The entire surface of the agar was streaked evenly. The plates were incubated at 37oC for 48 hours, after which the diameter of the zones of inhibition were measured using a Vernier caliper. The antifungal testing was done in seven replications. The experimental group has a very slight effect on the specimen with the average zone of inhibition of 0.537mm. The positive control (Nystatin) showed inconsistency in the zones of inhibition since the zones were changing from time to time. Normal saline solution has no change whether it was after 24 hrs. of incubation or after 48 hrs. So to determine the significant mean zone of inhibition (mm) difference between the experimental group and the positive control group, the independent t-test indicated there is significant difference of 1.15mm at t-value of 5.440 and a p-value equal to 0.000 which is lower the 0.05 p-value. The mean of + SD is 1.69+0.48.

             The researchers concluded that the coelomic fluid of the sea urchin (T. gratilla) has no antifungal effect on Candida albicans and recommended to the future researchers to try a study of similar kind using other species of sea urchin.



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Cebu Doctors' University - Main Library U1 An87b 2015 Fulltext Print Format

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