Giomen Probert L. Alayon,
Marygrace N. Amora,
Nessa Mae L. Cejas,
Erika Shannen A. Gabud,
Shara Mae A. Hinlayagan,
kevin Joe P. Ybañez,
Brian M. Denney
This study aimed to determine the inhibitory effect of Caimito (Chrysophyllum cainito) on the growth of Salmonella typhi. The experimental method used was randomized-controlled trial. Independent t-test was used in determining the significant difference between the positive control and the experimental set-up. Caimito bark samples were obtained from the Department of Agriculture. These were air-dried at room temperature under shade for one month. Then, these were powdered using a Hammer-Mill mechanical grinder and stored in zip lock plastic bags for storage and transport. An extract is prepared from this by measuring 125 grams of powdered Caimito bark and mixing it with 500 milliliters of distilled water. Alongside with Ceftriaxone, 25% (w/v) Caimito bark extract was placed in agar wells punched through the inoculated agar with Salmonella typhi and incubated at 37°C for 16-18 hours. The zone of inhibition is read and interpreted afterwards using a Vernier caliper.
The findings demonstrated a clear zone of inhibition around all the 16 agar wells of both the experimental set-up and the positive control, respectively. However, this does not automatically imply that the zone of inhibition formed by using the Caimito bark extract is significant because upon comparison with that of Ceftriaxone, the experimental set-up gave a narrower zone of inhibition with an average of 48.26 millimeters compared to Ceftriaxone which is 56.62 millimeters, suggesting that Salmonella typhi growth was inhibited using Caimito bark extract at 25% (w/v) prepared but not as effective as the positive control.
In conclusion, the results of this study points out that the Caimito bark extract at 25% (w/v) does indeed have a potential inhibiting effect on Salmonella typhi; but not as effective as Ceftriaxone in averting the proliferation of bacteria. Therefore, it is not significant enough to be used as an alternative for Ceftriaxone in withholding Salmonella typhi growth.
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