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Determination of the presence of Escherichia coli and comparison of MPN of microorganisms on seaweeds Caulerpa racemosa (sea grape) and Eucheuma cottonii sold in Carbon Market.


Christian Ace V. Bontilao,
Dawn Erika R. Mendez,
Rovy May D. Nablea,
Vinzville C. Sarmiento,
Schilazzi Palou Towe,
Vitaliano V. Fernandez

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
March 2016


          This study aimed to determine the presences of Escherichia coli on two edible seaweeds, Caulerpa racemosa (lato) and Eucheuma cottonii (guso) and determine the MPN (Most Probable Number) of other microorganisms on both seaweeds. The seaweeds samples were obtained from a major distributor in Carbon Market to which they were harvested and brought in from the province of Bohol to be sold.

          Based on the findings, this study found out the occurrence of bacterium E. coli on the seaweed species C. racemosa however found no presence at all in the seaweed E. cottonii. This was carried out with the usage of petrifilms for simple culturing method which enables the specific test for E. coli CFU. Utilizing 24 Petrifilms for each samples only three Petrifilms for E. coli culture on C. racemosa showed presence E. coli CFU whereas for the E. cottonii it showed no presence at all. The MPN was carried out and compared using serial dilution of nine nutrient broth test tubes for each sample. After the incubation, the outcome in both sample inoculums showed a bigger number of microorganisms.

          Based on the results, generally the seaweeds are found to be safe from E. coli contamination, however further studies for other possible pathogenic microorganisms are recommended as the MPN of other microorganisms is very high for both species.


1. Davis, J. G. , Kendall, P. . (2011, November 30). Preventing E. coli fro garden to plate.. . Retrieved from: http://extension.colostate.edu/topic-areas/nutrition-food-safety-health/preventing-e-coli-from-garden-to-plate-9-369/
2. Greene, A. . (2012, November 30). Staph: A-to-Z guide from diagnosis to treatment to prevention. . . Retrieved from: http://www.drgreene.com/articles/staph/
3. Hannah, F. , Cowie, P. R. . (2008, November 30). Evidence: The potential risks to human health posed by living, attached seaweeds and dead, beachcast material associated with sandy beaches: a preliminary report.. . Retrieved from: https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/291012/scho1009brgb-e-e.pdf
4. Inasea Enterprise, . (1995, November 30). Dried eucheuma spinossum seaweed. . . Retrieved from: http://inasea.en.ecplaza.net/dried-eucheuma-spinossum-seaweed--409591-3428733.html
5. Lofranco, L. B. M. , Belas, N. J. A. , Limpiado, M. K. D. , Superales, J. C. , Pascasio, M. K. I. O. , Daga, R. R. M. . Comparative analysis on the antibacterial effect of the different parts of lato (Caulerpa racemosa). 2007. Philippine Science High School, . Retrieved from: http://www.edu-sciece.com/2012/08/comparative-analysis-on-antibacterial.html
6. Kania, N. , Mayangsari, E. , Setiawan, B. , Nugrahenny, D. , Tony, F. , Wahyuni, E. S. , Widodo, M. A. . "The effects of eucheuma cottonii on signaling pathway inducing mucin synthesis in rat lungs chronically exposed to particulate matter 10 (PM10) coal dust." Journal of Toxicology. , 2013. Retrieved from: http://doi: 10.1155/2013/528146
7. Rabia, M. D. S. . " Cultivation of caulerpa lentillifera using tray and sowing methods in brackishwater pond." Environmental Sciences 4(1): 23-29, 2016. Retrieved from: http://www.m-hikari.com/es/es2016/es1-2016/51012.html
8. Blodgett, R. . (2009, November 30). Bacteriological analytical manual: Appendix 2: Most Probable Number from Serial Dilutions.. . Retrieved from: http://www/fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm109656.htm
9. Wagey, B. T. , Bucol, A. A. . "A brief note of lato (Caulerpa racemosa) harvest at Solong-on, Siquijor, Philippines." Budidaya Perairan 2(1): 2014. Retrieved from: http://ejournal.unsrat.ac.id/index.php/bdp/article/view/3793/3315

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U1 D48b 2016 Fulltext Print Format

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