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In vitro determination of the anthelmintic properties of three selected plants: Neem (Azadirachta indica), makahiya (Mimosa pudica), and malunggay (Moringa oleifera) leaves against Ascaris lumbricoides.


Fraulein Denise Arigo,
Anna Beatrice Briones,
Cindy Caballero,
Anton Miguel Garcia,
Theresa Jean Pragados,
Edmalyn Dehnave Visto,
Emmanuel A. Pardinan

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
October 2015


           This study was conducted to determine anthelmintic properties of three selected plants: Neem (Azadirachta indica), Makahiya (Mimosa pudica), and Malunggay (Moringa oleifera) leaves in the form of 50% aqueous test suspensions against Ascaris lumbricoides adult worms under laboratory conditions by gathering the mean percentage mortalities after twelve (12) replicates of randomized controlled experimental trials involving a total of four hundred and twenty (420) Ascaris lumbricoides adult worms, with thirty-five (35) subjects per replicate divided into five (5) groups of seven (7) worms randomly assigned to one experimental or control group, with the control groups consisting of Mebendazole as the positive control and 0.9% normal saline solution as the negative control. The anthelmintic property of each plant is determined by their capability to cause death to the Ascaris lumbricoides adult worms.

           The Azadirachta indica leaf extracts were able to cause death to an average of 92.86% of the test subjects, followed by Mimosa pudica having a mean percentage mortality of 80.95% and Moringa oleifera with 66.67% displaying that all three possess anthelmintic properties. Of the three, A. indica is the most promising especially when compared to the positive control. A. indica leaf extract is as powerful as the commercially available drug Mebendazole against Ascaris lumbricoides adult worms. It was also found that although both M. pudica and M. oleifera both posses' anthelmintic properties, these are weaker than Mebendazole in laboratory conditions.

           These findings imply that A. indica, M. pudica, and M. oleifera are candidates for becoming components of new, more natural, and cheaper anthelmintic drugs that may help in the imminent drug resistance of helminths against the currently available anthelmintic therapies on the market, after further and more in-depth study.


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