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The effect of polyphenol extract from Caulerpa racemosa var. lamourouxii against the growth of Staphylococcus aureus in laboratory conditions.

Author

Jeannine P. Arciga,
Rovill V. Carunungan,
Inahclaire Marie B. Miego,
Peavey Jan  G. Berame,
Gladys Marie T. Dinoy,
Marve D. Rabago,
Dan Anthony U. Bataan

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Biology
Publication Date
October 2015

Abstract

      Bacteria are present throughout time. The most common are the Staphylococcus group of bacteria. These bacteria are present in the environment abundantly and cause several complicated infections. Over 30 different types of staphylococci can infect humans, but most infections are caused by Staphylococcus aureus which causes superficial skin lesions (boils, styes) and localized abscesses in various tissues of the body. The infections caused by S. aureus are not limited to just one country but also globally. It affects many people around the world specially those who are immunosuppressed. Eliminating the bacterium is known to be impossible because of its abundance and multiple antibiotic resistances that are increasingly common. Thus, the use of cheap products like algae extracts is necessary to cure the infection. The extracts from Caulerpa racemosa var. lamourouxii, a green marine alga, contain metabolites that have antimicrobial properties. Caulerpa species are abundant, available throughout the year, and easily acquired in Cebu which makes the present study to determine the antimicrobial effect of C. racemosa polyphenol extract on the growth of S. aureus. The study specifically aimed to determine the mean Zone of Inhibition of C. racemosa extract on the following concentrations (20%, 40% and 60%).


       The following concentrations were established. The largest mean Zone of Inhibition was yielded by the 60% crude extract having a mean Zone of Inhibition of 8.33±0.71 millimeters. It was then followed by the 40% crude extract with a mean Zone of Inhibition of 7.84±0.84 millimeters. Lastly, the smallest mean Zone of Inhibition was seen on 20% crude extract with a mean Zone of Inhibition of 7.00±0.63 millimeters. The Positive control exhibited a large mean Zone of Inhibition which is 25.61±2.01 millimeters in diameter. However, the Negative control did not exhibit a Zone of Inhibition at all. The concentrations did not meet the Zone of Inhibition which is ideally >18 mm and there was a significant difference yielded by the positive control. The 60% concentration has a significant difference by 0.08 millimeters compared to 20% but not at 40%. Likewise, the 40% concentration significantly differs by 0.05 millimeters from that of 20%.


       Based on the results of the study, the researchers concluded that C. racemosa var. lamourouxii has no effect against Staphylococcus aureus and further investigations are required to fully understand its improvement and mode of action. On the other hand, the researchers recommended that the drug manufacturers should focus on other Caulerpa species that are proven to have an antimicrobial effect as a cheap and alternative drug. To the phycologist, further test the clinical features and benefits of Caulerpa species. To the seaweed farmers, expand the knowledge on Caulerpa species as they contain bioactive compounds which would be a key to improvement of business and information. Furthermore, future researchers should subject the algae into further experiments or studies to determine possible uses that are helpful to the community

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