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The effect of cogon grass (Imperata cylindrica) and carabao grass (Paspalum conjugatum) leaf extract on mortality of Aedes aegypti larvae.

Author

Pearl Mariam Calago,
Maria Ricka Dayao,
Philline Kent Roleda,
Cyrille Rosbelle Saquilabon,
Jerah Mae Tecson,
Johanna Anne Marie Wee,
Elvira A. Arong

Related Institution

College of Arts and Sciences - Biology Department - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Biology
Publication Date
October 2015

Abstract

      Dengue is one of the most common mosquito-borne disease. It is very rampant in the Philippines. Insecticides are sometimes inefficient in eradicating these insects. This study was conducted to determine the larvicidal potential of Imperata cylindrica and Paspalum conjugatum leaf extract at different concentrations namely: 20ppm, 40ppm and 60ppm. The study aimed to compare the percent mortalities across of the experimental and control groups. The experiment made use of 25 Aedes aegypti larvae per cup with 15 replicates per concentration. This research applied a completely randomized design (CRD) and was conducted in one of the Cebu Doctors' University CAS Laboratory. The dried grasses were macerated with ethanol. Ethanol was then taken out through evaporation with the use rotary evaporator. The crude extract was diluted with distilled water to acquire the desired concentration. After obtaining the results, it was processed and analyzed using the IBM SPSS version 22.


     The study showed 0% larvicidal effect by the extracts of Carabao and Cogon grass. Carabao grass contains organic phenolic compounds that are toxic to the larvae but the concentration used for the experimentation was not high enough to kill the larvae. Control groups were done and yielded expected results. Positive control, which was Baygon, showed 100% mortality while negative control which was distilled water, showed 0% mortality.


      Cogon grass did not show any mortality against the Aedes larvae in any of the given concentrations used due to the low amount of phenolic compounds that can cause larval death. Carabao grass only rendered to what can be observed as paralysis of the larvae in the first twenty four hours, larval movement was observed hours after. Higher concentrations of the Cogon grass is recommended and the use of second and third instar of Aedes larvae is suggested for future studies on potential larvicidal activity of the experimental plants.

References

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Cebu Doctors' University - Main Library U1 Ef36c 2015 Fulltext Print Format

 
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