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Determination of the approximate lethal dose of glycoside from Cucurbita Maxima flower extract.

Author

Jennifer O. Daruca,
Sittie Nadjmah C. Hadji Taha,
Lourlin Dynielle R. JIngco,
Rechell E. Lu,
Honeylene B. Paloma

Related Institution

College of Pharmacy - Cebu Doctors' University

Publication Information

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2013

Abstract



DESIGN: This study utilized the prospective-observational design to determine the Approximate Lethal Dose of Squash flower (Cucurbita Maxima) extract on Male Albino Mice.


SETTING: This study was conducted in the Pharmaceutical Laboratory of Cebu Doctors' University, Rm. 419, North Reclamation Area, Mandaue City, Cebu. It provides the mice a well ventilated spacious and adaptable climate.


PROCEDURES/INTERVENTIONS: The flowers were washed, air-dried, and chopped into pieces. 100 grams of the chopped flowers were weighed and placed in an Erlenmeyer flask, submerged with enough amount of 95% alcohol used as a solvent. After 24 hrs, prior to maceration the extractive was subjected to Reflux distillation until the alcohol evaporated and the marc was discarded. Stock solutions were prepared with different concentrations, 2% and 30% respectively. Administration of the computed dose of the extract was given intraperitoneally using Albino mice (20-25 grams). The Approximate Lethal dose was determined based on the given parameters (Respiratory Rate, Motor Activity, Eye observation, Circular Motor and Subject Test). The datas were recorded on an hour interval for 4 hours.


RESULTS: After gathering all the data of the five trials, there was a significant difference among the test solutions with increasing doses administered to the mice. This signified that the death of the mice was dose-related and pertaining to the result, there was an increase in number of death as the dose of the test solution was increased. The approximate lethal dose ranged from 39.81 mg/kg to 10000 mg/kg.


CONCLUSION: This study revealed that the glycoside extract present in the squash flowers had a significant toxic effects manifested by progressing death of the test animal.

Objectives



OBJECTIVE: The purpose of this study was to determine the Approximate Lethal Dose of Squash flower (Cucurbita Maxima) extract on Male Albino Mice.

References

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Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors' University - Main Library U8 D481 Fulltext Print Format

 
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