Author
Jagadeesh Babji Bejjanki,
Venkata Appala Durga Mahesh Bodda,
Harsha chanakya Garlapati,
Navya Suma Lakshmi Gudapati ,
Raviteja Pasarla,
Shalini Pathi,
Gnanasekhar Singaraju
Related Institution
Publication Information
Publication Type
Thesis/Dissertations
Publication Date
October 2014
Abstract
This is a descriptive study which aimed to determine the quantitative presence of Escherichia coli and other associated bacteria on the control panel buttons of Cebu Doctors' University elevators.
This study included a total of six samples where in two of which were randomly collected from each control panel of the three CDU elevators. Each sample was subjected to multiple dilutions of 1:10, 1:100 and 1:1000 respectively in order to obtain well isolated colonies. Each dilution was then subjected to presumptive tests for presence of fecal coliforms using Lauryl sulfate broth slants with Durham's inverted tube and then were transferred to E. coli and coliform count plate petrifilms. Each petrifilm plate later was incubated for 24-48 hours. The colonies found after incubation were quantitatively determined using the Quebec colony counter.
The colony count revealed that colonies of E. coli and other bacteria in each dilution were negative except for one colony of coliform bacteria from the sample taken on the panel of elevator 1 at a dilution of 1:10 whose mean was 0.5. However, the mean and the standard deviation for associated bacteria were different at different dilution of samples collected from the 3 CDU elevators. Elevator 2 turned out to have more bacterial colonies of non-fecal coliforms followed by elevator 1, and then elevator 3. Based from the data gathered, it is concluded that E. coli was absent in the buttons of the panels of the 3 CDU elevators. Associated bacteria noted include, a non-fecal coliform other than E. coli from 1:10 dilution of elevator 1 sample and several non-fecal coliforms collected from the panel buttons of the 3 CDU elevators. It is recommended that identification of the associated bacteria other than the fecal coliforms and their pathogenicity be conducted. The researchers also recommend to the future researchers to conduct a Comparative Study Between the Bacteria Present on Control Panels of Elevators and Bacteria Present on Hands of Elevator Users and a study on the Effectiveness of Certain Products in Reducing the Normal Bacterial Flora on the Control Panels of Elevators.
Objectives
References
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