Report an error   
Advanced Search

Quantitative determination of Escherichia coli with notes on other associated bacteria on the buttons of the panel of CDU elevetors.


Jagadeesh Babji Bejjanki,
Venkata Appala Durga Mahesh Bodda,
Harsha chanakya  Garlapati,
Navya Suma Lakshmi  Gudapati ,
Raviteja  Pasarla,
Shalini  Pathi,
Gnanasekhar  Singaraju

Related Institution

College of Arts and Sciences - Biology - Cebu Doctors' University

Publication Information

Publication Type
Thesis Degree
Publication Date
October 2014



This  is  a  descriptive  study  which  aimed  to  determine  the  quantitative  presence  of  Escherichia  coli  and  other  associated  bacteria  on  the  control  panel  buttons  of  Cebu  Doctors'  University  elevators.

This  study  included  a  total  of  six  samples  where  in  two  of  which  were  randomly  collected  from  each  control  panel  of  the  three  CDU  elevators.  Each  sample  was  subjected  to  multiple  dilutions  of  1:10,  1:100  and  1:1000  respectively  in order  to  obtain  well  isolated  colonies.  Each  dilution  was  then  subjected  to presumptive  tests  for  presence  of  fecal  coliforms  using  Lauryl  sulfate   broth  slants  with  Durham's  inverted  tube  and  then  were  transferred  to  E. coli  and  coliform  count  plate  petrifilms.  Each  petrifilm  plate  later   was  incubated  for  24-48  hours.  The  colonies  found  after  incubation  were  quantitatively  determined  using  the  Quebec  colony  counter.

The  colony  count  revealed  that  colonies  of  E. coli  and  other  bacteria  in  each  dilution  were  negative  except  for  one  colony  of  coliform  bacteria  from  the  sample  taken  on  the  panel  of  elevator  1  at  a  dilution  of  1:10  whose  mean  was  0.5.  However,  the  mean  and  the  standard  deviation  for  associated  bacteria  were  different  at   different  dilution  of  samples  collected  from  the  3  CDU  elevators.  Elevator   2  turned  out  to  have  more  bacterial  colonies  of   non-fecal  coliforms  followed  by  elevator 1,  and  then  elevator  3.  Based  from  the  data  gathered,  it  is  concluded  that  E. coli  was  absent  in  the  buttons  of  the  panels  of  the  3  CDU  elevators.  Associated  bacteria  noted  include,  a  non-fecal  coliform  other  than        E. coli  from  1:10  dilution  of  elevator  1  sample  and  several  non-fecal  coliforms  collected  from  the  panel  buttons  of  the  3  CDU  elevators.  It  is  recommended  that identification  of  the  associated  bacteria  other  than  the  fecal  coliforms  and  their  pathogenicity  be  conducted.  The  researchers  also  recommend  to  the  future  researchers  to  conduct  a  Comparative  Study  Between  the  Bacteria  Present  on  Control  Panels  of  Elevators  and  Bacteria  Present  on  Hands  of  Elevator  Users  and  a  study  on  the  Effectiveness of  Certain  Products  in  Reducing  the  Normal  Bacterial  Flora  on  the  Control  Panels of  Elevators.




1. References Ashgar, S S, El-Said, H M. "Pathogenic bacteria associated with different public environmental sites in Mecca City. " Open Journal of Medical Microbiology 2(4): 133-137, 2012. Retrieved from: http://doi:10.4236/ojmm.2012.24020
2. Bauman,, R W. Microbiology. San Francisco: CA: Pearson Education,
3. Bures, S , Fishbain, J T, Uyehara, C F, Parker, J M, Berg, B W. "Computer keyboards and faucet handles as reservoirs of nosocomial pathogens in the intensive care unit. " American Journal of Infection Control 28(6): 465-701, October 2014. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/11114617
4. Datta, P , Rani, H , Chander, J , Gupta, V . "Bacterial contamination of mobile phones of health care workers. " Indian Journal of Medical Microbiology 27(3): 279-281, . Retrieved from: http://doi:10.4103/0255-0857.53222.
5. Edberg, S C, Rice, E W, Karlin, R J, Allen,, M J. "Escherichia coli: The best biological drinking water indicator for public health protection. " Journal of Applied Microbiology 88, 106s-116s, October 2014. Retrieved from: http://onlinelibrary.wiley.com/store/10.1111/j.1365 -2672.2000.tb05338.x/asset/j.1365-2672.2000.tb05338.x.pdf?v=1&t=i57je6ml &s=940d8e2fcc80918c98b2b5beb84aee9f2ebaca45
6. Harbi, W . (2014, November 30). Escherichia coli.. . Retrieved from: https://www. prezi.com/alkaz9mjnmio/escherichia-coli/201
7. Ismail, K S. "A preliminary study of bacterial contamination from elevators" International Journal of Scientific Research 2(10): 1-2, March 2014. Retrieved from: http://theglobaljournals.com/ijsr/file .php?val=October_2013_1380979676_205f5_149.pdf
8. Jacobs, J A, Van Ranst, M . " Biometric fingerprinting for Visa application: Device and procedure are risk factors for infection transmission. " Journal of Travel Medicine 15(5): 335-343, . Retrieved from: http://doi:10.1111/j.1708-8305.2008. 00232.x
9. Joshaline, M C, Subathra, M , Shyamala, M , Padma, S , Rekha., . "Automated Teller Machine (ATM)- A ”pathogen city”- A surveillance report from locations in and around Madurai City, Tamil Nadu, India. " International Journal of Public Health Science 3(1): 51-56, January 2015. Retrieved from: http://www.iaesjournal.com/online/index.php/IJPHS/article/download/5155/ 2759
10. Kenny, T . (2013, November 30). E. coli and VTEC 0157. . Retrieved from: www.patient.co.uk/health/E.-Coli-and-VTEC-0157.htm.
11. Marsden, R . (2013, November 30). Solid-surfaced infection control computer keyboard. . Retrieved from: www.cleankeys.nl/WhitePaper.pdf
12. Noskin, G A, Stosor, V , Cooper, I , Peterson, L R. " Recovery of vancomycin resistant enterococci on fingertips and environmental surfaces. " Infection Control and Hospital Epidemiology 16(10): 577-581, October 2014. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed /8568202
13. Rusin, P , Maxwell, S , Gerba, C . " Comparative surface-to-hand and fingertip-to-mouth transfer efficiency of Gram-positive bacteria, Gram- negative bacteria, and phage. " Journal of Applied Microbiology 93(4): 585 -592, October 2014. Retrieved from: http://onlinelibrary.wiley.com /store/10.1046/j.1365-2672.2002.01734.x/asset/j.1365-2672.2002.01734.x.pdf? v=1&t=i4jvn3v2&s=81a8f1698985b7d05fff7a58669d645cd17a2cbd
14. Todar, K . (2013, November 30). Pathogenic E. coli. . . Retrieved from: http://www.textbookofbacteriology.net/e.coli.html
15. Yazah, A J, Yusuf, J , Agb, A J. "Bacterial contaminants of Nigerian currency notes and associated risk factors" Research Journal of Medical Sciences 6(1): 1-6, January 2015. Retrieved from: http://docsdrive.com/pdfs/medwelljournals/rjmsci/2012/1-6.pdf

Physical Location

LocationLocation CodeAvailable FormatAvailability
Cebu Doctors U1 Q25b Fulltext Print Format

©2022 HERDIN PLUS. All rights reserved. | Contact Us | Keep up to date