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Molecular detection and serotyping of dengue virus in Metro Manila, Philippines.


Ronald R. Matias,
Ma. Giselle V. Espiritu,
Corazon C. Buerano,
Filipinas F. Natividad,
Akira Igarashi,

Related Institution

Institute of Biology/College of Science - University of the Philippines

Research and Biotechnology Division - St. Luke's Medical Center

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Publication Information

Publication Type
Publication Sub Type
Journal Article, Original
National Academy of Science and Technology Philippines.
Publication Date

Publication Type
Publication Sub Type
Journal Article, Original
St. Luke's Journal of Medicine
Publication Date
January-June 2010


Dengue virus infection is a persistent health problem in the Philippines. Surveillance and diagnosis have been limited to recognizing the clinical symptoms. In this study, a reverse transcription-polymerase chain reaction (RT-PCR) assay complemented with virus isolation was used to detect and isolate dengue virus from sera of patients admitted to different medical institutions during the period 1995-1998. RT-PCR was done directly on serum samples. Additionally, serum samples were inoculated on Aedes albopictus cells and RT-PCR was done on infected culture fluid or on RNA extracted from infected cells. Dengue virus was detected and isolated in 95 out of 738 serum samples. Serotyping of the virus indicated the prevalence of Den 2 and Den 3.


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