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Prevention of reperfusion injury in acute leg ischemia: An animal study.


Victor O. Hofilena,
Gisel T. Catalan,
Renato A. Villanueva,
Florimond A. Garcia

Related Institution

Division of Cardiovascular Surgery, Section of Experimental Surgery and Research and the Peripheral Vascular Section - Philippine Heart Center

Publication Information

Publication Type
Publication Sub Type
Journal Article, Original
Philippine Journal of Cardiology
Publication Date
July-September 2000


OBJECTIVE: The purpose of this study to determine the effects of distal venous washout during reperfusion injury in experimentaly induce acute leg ischemia.

BACKGROUND: Reperfusion injury accounts for deaths during revascularization procedures for acute arterial occlusion.

METHODS: Thirty-two adult rabbits were equally divided into a control and an experimental (Distal Venous Washout-DVW) group. Both groups underwent ligation of the left common femoral artery. After six hours, 10 cc of venous blood was extracted in the DVW group after releasing the arterial ligature and was given an equal amount of saline. For both groups venous blood was measured for pH, potassium, calcium and hematocrit levels prior to leg ischemia, then at 5, 30, and 60 minutes after reperfusion.

RESULTS: There was a significant drop in pH in the control group without distal venous washout 5 minutes after hind limb reperfusion (7.23 versus 7.30 p=0.011). Potassium levels were lower in the control group 1 hour after reperfusion (3.67 versus 4.05 p=0.045). Hematocrit levels were higher in the control group than the experimental group noted on the 5th minute after reperfusion (0.37 versus 0.28 p=0.004).

CONCLUSION: Distal venous washout results in decreases acidosis in the immediate reperfusion period.(Author)

Physical Location

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Philippine Council for Health Research and Development Fulltext Print Format
Philippine Council for Health Research and Development Fulltext pdf (Request Document)

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