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The role of docosahexaenoic acid in milk formulas on the spatial working capacity of young white mice.


Philip Conrad M. Acop

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Publication Information

Publication Type
Publication Sub Type
Journal Article, Original
Philippine Journal of Pediatrics
Publication Date
October-December 2005


OBJECTIVE: To determine if male mice maintained on DHA fortified milk formula develop better spatial working capacity than male mice maintained on non-DHA fortified milk formula.

DESIGN: Randomized double-blind placebo-controlled experimental study.

SUBJECTS: Forty-five 5-week old male white mice.

METHODS: The subjects were randomly assigned to one of the three groups. Subjects were fed standard rodent diets supplemented with DHA-fortified milk formula, non DHA-fortified milk formula and standard rodent diet. Spatial learning using a T and 8-arm radial maze was then determined using the variables of time and errors made prior to task completion after handling and training. Trial runs were done every three days.

MEASURES: Repeated analysis of variance was used to determine differences in task completion between treatment groups. Independent T-test was done to determine when the treatment groups differed. All statistical tests used to analyze the data were two-tailed at alpha = 0.05 level of significance.

RESULTS: Results revealed not significant difference for DHA fortified and non-DHA groups from the placebo group when time to complete the task was considered. The group maintained on a diet supplemented with a non-DHA fortified milk formula had less error days compared to the placebo group.

CONCLUSION: These results suggest that DHA fortified milk formulas do not improve spatial working capacity in mice. (Author)


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